用噬菌体多肽库筛选日本血吸虫表膜抗原的模拟表位  

Immunoscreening Mimic Epitope of Schistosoma Japonicum Membrane Antigen by Phage Display Peptide

作　　者：秦志强[1] 曾庆仁[1] 易新元[1] 周东明[1] 张顺科[1] 曾宪芳[1] 

机构地区：[1]中南大学湘雅医学院寄生虫学教研室,长沙410078

出　　处：《上海免疫学杂志》2003年第3期181-183,189,共4页Shanghai Journal of Immunology

基　　金：湖南省血防世行贷款资助项目 (No 2 0 0 0 0 4)

摘　　要：为探索可用于诊断的模拟表膜抗原。采用纯化的兔抗表膜抗原IgG作探针 ,免疫筛选噬菌体随机十二肽库 ,经 3轮生物淘洗后 ,随机挑选 30个噬菌体克隆 ,用ELISA检测其与筛选抗体的特异性结合 ,选择两个阳性克隆进行DNA序列测定 ,并用斑点ELISA比较检测正常人和日本血吸虫病患者血清各 10份。结果显示 ,随机挑选的 30个克隆中有 9个噬菌体克隆与筛选的抗体有特异性的结合反应。DNA测序结果显示 ,两个阳性噬菌体克隆 (携带的抗原表位 )所演绎的氨基酸序列与GenBank已知的氨基酸序列无同源性。In order to explore an membrane mimotope in the diagnostic antigens of Schistosoma Japonicum specific IgG was purified from polyclonal antibodies (polyAb) which was against membrane of Schistosoma japonicum (SjMAg),and polyAb were used as probe to immunoscreen phage display random 12 peptides library Thirty clones were randomly selected and examined by ELISA with SjMAg immune serum The positive clones were sequenced and were used in the dot ELISA with 10 sera of schistosomiasis patients and normal human,respectively The results showed that nine phage clones displayed high affinity and specificity to anti membrane antibodies of Schistosoma japonicum .The sequence of two peptides (Q2 and Q6) showed no homology with other sequence in the GenBank In dot ELISA with Q2 and Q6,8 and 7 of 10 sera samples from schistosomiasis patients showed positive reaction,respectively,and sera from 10 normal persons all showed negative reaction with these two short peptides

关 键 词：日本血吸虫病 表膜抗原 模拟表位 噬菌体肽库 免疫筛选 

分 类 号：R392.11[医药卫生—免疫学] R53[医药卫生—基础医学]