从核糖体DNA ITS区序列研究甘蔗属及其近缘属种的系统发育关系  

Phylogenetic Relationships of Saccharum and Related Species Inferred from Sequence Analysis of the nrDNA ITS Region

作　　者：陈辉[1] 范源洪[1] 向余颈攻[2] 蔡青[1] 张亚平[2] 

机构地区：[1]云南省农业科学院甘蔗研究所,云南开远661600 [2]中国科学院昆明动物所细胞与分子进化开放研究实验室,云南昆明650223

出　　处：《作物学报》2003年第3期379-385,共7页Acta Agronomica Sinica

基　　金：云南省自然科学基金重点项目资助 ( 96C0 0 5 Z)

摘　　要：测定了甘蔗属及其近缘属的 13个种共 4 3个个体和 1个橡草 (Pennisetum schumach)外群的核糖体 DNA中的内转录间隔区 (ITS)及 5 .8S r DNA基因的序列。结果表明 :甘蔗属及其近缘属种的 ITS区 (含 ITS1,5 .8S r DNA和ITS2 )序列的长度范围为 5 89～ 5 91bp,变异位点为 14 0个 ,信息位点为 6 0个 ;其中 ,ITS1和 ITS2的长度范围分别为 2 0 5～ 2 0 8bp和 2 16～ 2 2 0 bp,变异位点分别为 6 4和 6 8个 ,信息位点分别为 33和 2 4个 ;5 .8S r DNA长度范围为 16 4 bp,变异位点为 8个 ,信息位点为 3个。以狼尾草属的橡草 (P.schumach)作为外群 ,运用 PAUP4 .0 b软件构建的系统发育 NJ树表明 :浙江果蔗 (R4 5 )应属于热带种 (S. officinarum ) ;河王八属与蔗茅属的亲缘关系较近 ,各自的金猫尾种与蔗茅种、河王八种与滇蔗茅种分别交叉聚在 2个不同的小分支中 ;斑茅 (S.arundinaceum或 E.arundinaceum)具有其他属不同的特异位点 ,是距甘蔗属较远的一个独立分支 ,从分子水平上提出了斑茅并不属于甘蔗属的观点 ,但其是否属于蔗茅属 ,还是应当立为单种属则需进一步的研究论证。The internal transcribed spacer(ITS) regions of nuclear ribosomal DNA from 14 species of Saccharum and related genera were sequenced. The regions ranged in length from 589 to 591bp, with ITS1(205~208bp)slightly shorter than ITS2(216~220bp). Of the 591bp aligned positions, there were 140 variable sites(64 in ITS1, 68 in ITS2, 8 in 5.8S region), and 60 informative sites(33 in ITS1, 24 in ITS2, 3 in 5.8S region). The neighbor joining tree based on the ITS sequences analysis with P. schumach as outgroup indicated that Zhejiang fruit sugarcane should belong to S. officinarum. N. fallax and E. fulvus, N. porphyrocoma and E. rockii should be placed in a same genus. Arundinaceum has different variable sites from other species, it is an independent clade isolated from Saccharum and Erianthus . The present analysis demonstrated that Arundinaceum should not belong to Saccharum , which is consistent with the results from morphology and cytology.

关 键 词：核糖体DNA ITS区 甘蔗属 近缘属种 系统发育 

分 类 号：S566.1[农业科学—作物学]