骨膜间质干细胞移植时机的实验和临床研究  

Experimental and clinical study on transplanted time of periosteal mesenchymal stem cell

作　　者：徐栋梁[1] 李佛保[1] 符气祯[1] 黄纲[1] 杨忠汉[1] 韩士英[1] 

机构地区：[1]中山大学附属第一医院骨科,广州市510080

出　　处：《中华显微外科杂志》2003年第2期116-118,共3页Chinese Journal of Microsurgery

基　　金：广东省科委重点项目基金资助 (960 2 2 0 50 - 0 3)

摘　　要：目的　研究骨膜间质干细胞异体移植时机。　方法　根据骨缺损内纤维组织生长时间的不同 ,将从大鼠骨膜分离培养的细胞分别分批植入 ,在植入的第 2、3、4和 8周时动态观察其成骨量 ,即骨小梁体积比 ;另将幼儿骨膜间质干细胞悬液 ,移植于 2 6例长管状骨干骨折 2周后的缺损内 ,观察骨折临床愈合的时间。　结果　大鼠骨缺损后 2周以内移植干细胞其骨小梁体积比大 ,愈合快 ,2周以后与对照组比无显著差异 ;临床应用 9个月内病人骨折缺损愈合 15例 ( 5 7 7% ) ,骨不愈合者 11例( 4 2 3 % )。　结论　大鼠骨缺损 2周以内移植干细胞其疗效好 ,大鼠实验和临床上骨缺损 2周以后移植骨膜间质干细胞不能明显促进骨缺损愈合。Objective Investigating transplanted time of periosteal mesenchymal stem cell (PMSC) Methods Rat PMSCs were allografted into rat tibia defects made in different time of granulation Then the quantity of formatted bone,i.e.,trabecular volumm rate (TVR),in the defects were measured in histology and bone histomorphometry by 2,3,4 and 8 weeks respectively after transplantation The long bone draft fracture defects of patients were allografted with PMSC of youth children after 2 week of fracture Results TVR was higher and the defects healed more fast in those rats transplanted PMSCs within 2 weeks after the defect were made However there was insignificant difference between the groups of PMSC transplantation was carried out after two weeks of the defect The fractured defects were healed in 15 cases (57 7%) and were not healed in 11 cases (42 3) after 9 months of transplantation Conclusion That rat bone defects were transplanted PMSC within 2 weeks showed expected bone formed effect and insignificant effect on accelerating defect healing by PMSC transplantation in rats or patients after 2 weeks.

关 键 词：骨缺损 骨膜间质干细胞移植 骨组织工程化材料 骨小梁体积比 动物实验 

分 类 号：R687[医药卫生—骨科学]