聪圣胶囊对小鼠脑缺血损伤后细胞内游离钙含量的影响  

Effect of Congsheng Capsule on Intracellular Calcium Concentration in Mice after Ischemic Cerebral Injury

作　　者：赵玲[1] 徐秋萍[2] 李林[1] 

机构地区：[1]首都医科大学宣武医院药理研究室北京脑老化重点实验室,北京100053 [2]北京中医药大学药理教研室

出　　处：《中国中西医结合杂志》2003年第4期281-283,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家"九五"攻关课题 (No .96 90 6 0 9 0 3)

摘　　要：目的 :研究聪圣胶囊对小鼠脑缺血再灌注后脑组织突触体及拟缺血损伤神经细胞内〔Ca2 + 〕i的影响 ,分析聪圣胶囊抗脑缺血损伤的作用机制。方法 :采用Fura 2 /AM双波长荧光分光光度法 ,检测小鼠脑组织突触体和胎鼠神经细胞悬液内〔Ca2 + 〕i。结果 :聪圣胶囊 3g/kg和 9g/kg灌胃给药可减轻小鼠脑缺血再灌注后突触体内钙超载程度 ;聪圣胶囊含药血清可抑制低糖低氧 1h及高浓度 (2 0 0 μmol/L)谷氨酸引起的神经细胞内钙的升高。结论 :聪圣胶囊可通过减轻脑缺血损伤后钙超载程度来发挥抗脑缺血作用。Objective:To investigate the effect of Congsheng Capsule (CSC) in intracellular Ca 2+ concentration in cerebral intra-synaptosomes of ischemia/reperfusion mice and analogous ischemic injured neurons to estimate the mechanism of CSC in antagonizing ischemic cerebral injury. Methods:The concentration of Ca 2+ in cerebral intra-synaptosomes of mice and in neurocyte suspension of fetal mice were determined using Fura-2/AM double wavelength fluorescence spectrophotometer. Results:CSC 3 g/kg and 9 g/kg through gastrogavage could relieve the degree of calcium overloading of intra-synaptosomes in mice after cerebral ischemia/reperfusion. The CSC containing serum could inhibit the elevation of Ca 2+ content in neurocytes induced by 1 hour hypoxia and glutamate of high concentration (200μmol/L). Conclusion:CSC plays a role in antagonizing ischemic injury through relieving the calcium overloading after cerebral ischemia.

关 键 词：脑缺血 学习记忆障碍 聪圣胶囊 中药制剂 游离钙 动物实验 

分 类 号：R285.5[医药卫生—中药学]