毛大丁草根止咳化痰活性成分的研究  

The Antitussive and De-sputum Essential Substance from the Root of Gerbera piloselloides and Its Analytical Compositions

作　　者：唐小江[1] 黄华容[1] 方铁铮[1] 杨翠平[1] 张援[1] 吴邦华[2] 许实波[1] 

机构地区：[1]中山大学,广东广州510275 [2]广东省职业病防治院,广东广州510300

出　　处：《中国中药杂志》2003年第5期426-429,共4页China Journal of Chinese Materia Medica

基　　金：广东省中医药局科研课题 (10 10 2 8)

摘　　要：目的 :研究毛大丁草根的止咳化痰作用及其有效部位的化学成分。方法 :将毛大丁草根用乙醇提取 ,乙酸乙酯萃取 ,经硅胶柱层析得到红色挥发性油状成分 (G4)。用GC MS技术 ,对其化学成分进行分析和鉴定 ,计算各组分的相对含量。用氨水刺激法进行小鼠止咳试验 ,用酚红排泄法进行化痰试验。结果 :G4共分离出 4种主要成分 ,分别为石竹烯 (15 .16 0 % )、氧化石竹烯 (2 1.14 0 )、环氧化马儿铃烯 (2 .6 73% )、6 乙酰基 2 ,2 二甲基 8(3 甲基 2 丁烯基 ) 2H 苯并呋喃 (6 0 .0 77% )。G4灌胃 2 0 0 0mg·kg-1时对小鼠止咳作用的R值为 16 2 .2 6 ,属显效 ;灌胃2 0 0 0和 10 0 0mg·kg-1时的化痰率分别 189.16 %和 187.15 %。结论 :首次从毛大丁草根中分离筛选出有良好止咳化痰活性成分 。Objective: To study the chemical constituents of the essential substance from the root of Gerbera piloselloides and its antitussive and de sputum effects. Method: The essential substance (G 4) was extracted from the root by alcohol and ethyl acetate, then it was separated by silica gel column eluted by the mixture of ethyl acetate and petroleum ether (5∶95). Its chemical components were separated and identified by GC MS. Its antitussive and de sputum effect was tested by mice. Result: 4 main peaks were separated and identified by GS MS. They are β caryophyllene (15.160%), caryophyllene oxide (21.140%), aristolenepoxide (2.673%) and 6 acetyl 2,2 dimethyl 8(3 methyl 2 butenyl) 2H chromoene (60.077%) respectively. Its antitussive and de sputum effect was prominent when the mice was given G 4 2 000 mg·kg -1 ig. Conclusion: Itis the first time that the antitussive and de sputum essential substance was separated from the root of Gerbera piloselloides and its main compositions were analyzed.

关 键 词：毛大丁草 根 中药材 止咳化痰作用 有效成分 硅胶柱层析法 动物实验 

分 类 号：R284.1[医药卫生—中药学] R285.5[医药卫生—中医学]