三七总皂苷及其单体对人骨髓造血祖细胞增殖作用的研究  

Proliferation of hematopoietic progenitor cells from human bone marrow induced by panax notoginosides(三七总皂苷)

作　　者：郑茵红 高瑞兰 朱大元[2] 钱煦岱 牛泱平 

机构地区：[1]浙江中医学院附属医院,浙江杭州310006 [2]中科院上海药物研究所,上海200031

出　　处：《中国中西医结合急救杂志》2003年第3期135-137,共3页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：国家自然科学基金资助项目 ( 3 0 0 70 93 3 )

摘　　要：目的 :了解三七总皂苷 (PNS)及其单体 (Rg1、Rb1、Re+R1 )对造血祖细胞的增殖作用 ,以筛选出促进造血的有效单体及其浓度效应点。方法 :采用骨髓粒系、红系祖细胞 (CFU GM和 CFU E)半固体培养集落形成法 ,观察不同浓度的 PNS及其单体 (Rg1、Rb1、Re+R1 )对人造血祖细胞的刺激增殖作用。结果 :1 PNS对造血祖细胞有增殖作用 ,浓度为 8、4 0和 1 0 0 mg/ L时 ,CFU GM集落产率均明显高于对照组 (P均 <0 .0 1 ) ,提高率分别为 (32 .5± 6 .8) %、(5 9.3± 8.7) %和 (37.0± 1 .6 ) % ;CFU E集落增高在 PNS浓度为4 0 m g/ L时最为明显 ,提高率为 (33.3± 7.3) % (P<0 .0 1 )。 2 PNS单体对造血祖细胞有增殖作用 ,Rg1浓度为8和 4 0 mg/ L 时 ,CFU GM集落产率明显高于对照组 (P均 <0 .0 1 ) ,提高率分别为 (33.5± 3.8) %和 (5 9.7±4 .7) % ;Rb1浓度 4 0 m g/ L 时 ,CFU E集落产率明显高于对照组 (P<0 .0 1 ) ,提高率为 (4 7.3± 6 .3) % ;单体Re+R1对 CFU GM和 CFU E均无明显作用。结论 :1 PNS能够促进人骨髓粒系、红系造血祖细胞的增殖 ;2三七 Rg1和Objective: To investigate the proliferation efficiency of both panax notoginosides and monomers on hematopoietic progenitor cells of colonyforming unitgranulocyte macrophage (CFUGM) and colonyforming uniterythroid(CFUE). Methods: Human bone marrow cells were incubated with semisolid culture systems in the presence of vary concentration of PNS and its monomers of Rg1, Rb1, Re+R1 for seven days. The increasing rate of CFUGM and CFUE colony formation induced by PNS was estimated in vitro. Results: ①The hematopoietic progenitor cells from bone marrow were proliferated in the response to stimulation of PNS. After being incubated with PNS 8, 40 and 100 mg/L, the increasing rates of CFUGM colony forming were (32 5±6 8)%, (59 3±8 7)% and (37 0±1 6)% respectively (all P <0 01 ). The CFUE colonies was raised by (33 3±7 3)% ( P <0 01) at the concentration of PNS 40 mg/L. ②The monomer Rg1 could raise the colony formation of CFUGM with increasing rate of (33 5±3 8)% and (59 7±4 7)% (both P <0 01) when the cells were exposed to Rg1 8 and 40 mg/L respectively. While 40 mg/L of Rb1 could elevate CFUE colony by (47 3±6 3)% ( P <0 01). However, the monomer Re+R1 did not raise the colony formation of both CFUGM and CFUE. Conclusion: PNS can promote the proliferation on hematopoietic progenitor cells of CFUGM and CFUE. Meanwhile, Rg1 and Rb1 isolated from PNS are the effective monomer to enhance the proliferation on CFUGM and CFUE respectively.

关 键 词：三七总皂苷 单体 人造血祖细胞 细胞增殖 骨髓粒系 骨髓红系 

分 类 号：R285.5[医药卫生—中药学]