雌激素对大鼠认知能力的影响(英文)  

The effect of 17-beta-estrogen on the capability of rat 's cognition

作　　者：徐杰[1] 王书汉[1] 戴忠辉[2] 

机构地区：[1]中山大学中山医学院人体解剖学教研室,广东省广州市510080 [2]蚌埠医学院组胚教研室,安徽省蚌埠市233003

出　　处：《中国临床康复》2003年第10期1494-1495,共2页Chinese Journal of Clinical Rehabilitation

基　　金：广东省中医药局科研项目(402013)~~

摘　　要：目的通过定位航行实验结果、空间搜索试验结果、CA1区锥体细胞树突棘的观察,探讨雌激素对去势雌性大鼠认知能力的影响。方法4月龄雌性SD大鼠28只(其中10只用于Golgi-Cox组织染色),体质量200g,经随机抽签法分用药组犤雌二醇肌注0.25mg/(kg·d)犦和对照组各14只,每组有9只动物用Morris水迷宫技术测试大鼠学习记忆能力,另5只在用药10d后用Golgi-Cox染色,观察海马CAl区锥体细胞树突棘的变化。结果(1)用药10d后,定位航行实验显示:后4个时间段用药组和对照组潜伏期分别为(9±3)和(14±4)s,差别有显著性意义(t=2.981,P<0.05);Golgi-Cox染色显示对照组中段树突棘数为19.5±2.3,用药组为25±3,差别有显著性意义(t=3.805,P<0.05)。(2)用药30d后,用药组和对照组潜伏期分别为(17±6)和(36±9)s,在原有平台象限游泳路线长度占总长度42%±4%和32%±5%;跨越平台次数分别为3.1和1.6次,差别有显著性意义(t=4.682,4.762,P<0.01)。结论雌二醇能增强大鼠认知能力,特别是记忆能力。Aim On basis of the results of the orient r unning test and the spatial search test,together of the observation on the dendritic spines of the pyramidal cells in the CA 1 zone of hippocampus,to discuss the e ffect of estrogen on an emasculated female rat.Methods 28SD rats(4-month-old,female,200g )were divided into 2groups by sign ran domly:estrogen treatment group0.25mg /(kg·d),imand control group.l0days later,9ra ts in each group were tested in Morris Water Maze for t heir learning memory.The other 5in e ach group were stained with Golgi-Cox te chnology to observe the structure of the dendritic spine.Results(1)After 10days,the orient running test showed that the escape latency was(14±4)s in the control group and(9±3)s in the treatment group in the later 4interv als,with a significant difference(t=2.981,P<0.05).Gogli-Cox showed that the dendriti c spines in the estrogen group(19.5±2.3)were significantly increased(t=3.805,P<0.05),contrast to the control group(25±3).(2)One month later,the escape latency was(36±9)s in the control group and(17±6)s in the treatment group.The estrogen increased 32%±5%and 42%±4%of the total swim path lo-cated within the platformquadrant of pool in the control group and the tre atment group respectively(t=4.682,P<0.01).The times crossing the platform was 3.1and 1.6separately,with a sig nificant difference(t=4.762,P<0.01).Conclusion The results suggests that estrogen h as an effect on the central nervous system and can enhan ce the learning memory of female rats.

关 键 词：雌激素 认知能力 雌二醇 记忆能力 去势雌性大鼠 

分 类 号：Q579.13[生物学—生物化学] R338.6[医药卫生—人体生理学]