肝素在高糖影响大鼠腹膜间皮细胞的增殖及其表达白细胞介素-1中的作用  

Effects of heparin on the role of high glucose in rat peritoneal mesothelial cell's proliferation and its expression of interleukin- I.

作　　者：刘作海[1] 王钢[1] 车文体[1] 伍靖武[1] 眭维国[1] 晏强[1] 张清勇[1] 

机构地区：[1]广州军区肾病研究所,中国人民解放军第181医院肾脏科,桂林541002

出　　处：《中国血液净化》2003年第5期260-263,共4页Chinese Journal of Blood Purification

摘　　要：目的初步观察肝素在高糖影响大鼠腹膜间皮细胞(Rat peritoneal mesothelial cells,RPMC)细胞增殖及其表达白细胞介素-1(Interleukin-1,IL-1)中的作用,从而为持续性非卧床腹膜透析(Continuous ambu-latory peritoneal dialysis,CAPD)中肝素的临床应用打下一定的理论基础。方法 建立RPMC的体外培养;并用四四氮唑蓝(MTT)细胞增殖实验和酶联免疫吸附方法(ELISA),来分析肝素对高糖(3.86％葡萄糖)影响RPMC增殖及其分泌IL-1的作用。结果 3.86％葡萄糖能显著抑制RPMC的增殖,而1.25U/ml肝素能部分逆转高糖对RPMC增殖的抑制作用(P<0.01);3.86％葡萄糖能显著增加RPMC表达IL-1β,而1.25U/ml肝素能部分减少高糖增加RPMC表达IL-1β的作用(P<0.01)。结论 本文结果提示,肝素可能通过逆转高糖对RPMC的增殖抑制作用及减少高糖增加RPMC表达促炎细胞因子IL-1的作用,而延缓CAPD相关性腹膜纤维化的发生,最终有益于CAPD进行。Objective To investigate the effect of heparin on inhibition of cell proliferation as well as on expression of interleukin- 1(IL- 1) in RPMC (rat peritoneal mesothelial cells) by high glucose, so as to provide some theoretic basis for the clinical use of heparin during continuous ambulatory peritoneal dialysis ( CAPD) . Methods RPMC were cultured in vitro and MTT method was processed to observe the effects of both high glucose (3.86%) and heparin (1.25U/ml) on cell proliferation. In addition, ELISA analysis were performed to investigate the change of IL- 1 in the culture muli. Results High glucose of 3.86% could significantly inhibit cell proliferation as well as induce the expression of IL- 1β in RPMC (P < 0.01), which could be partially reversed by heparin of 1.25U/ml( P < 0.01). Conclusion Heparin could reverse both inhibition of cell proliferation and induction of pro - inflammatory cytokine IL - 1 mediated by high glucose in peritoneal mesothelial cells, and delay the occurrence of CAPD associated peritoneal fibrinosis and being beneficial to the process of CAPD.

关 键 词：肝素 高糖 大鼠 腹膜间皮细胞 细胞增殖 细胞 白细胞介素—1 持续性非卧床腹膜透析 

分 类 号：R459.5[医药卫生—治疗学]