深低温保存犬同种主动脉用于腔静脉移植的研究  

Experimental study on cryopreserved aortic homograft interposed in the inferior vena cava in canine

作　　者：吴继敏[1] 汪忠镐[1] 张鸿坤[1] 李鸣[1] 金炜[1] 封华[1] 陶淑珍[1] 

机构地区：[1]浙江大学医学院附属第一医院血管外科

出　　处：《中华实验外科杂志》2003年第6期532-533,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目 (30 1 70 91 8)

摘　　要：目的　探讨同种异体主动脉用于腔静脉重建的可行性。方法　将 15条杂种犬等分为 3组。Ⅰ组用深低温保存的主动脉间置移植于犬肾下下腔静脉 ,长 4cm ;Ⅱ组采用新鲜的主动脉移植 ;Ⅲ组采用涤纶人工血管移植。 2个月后获取血管 ,观察其通畅情况 ,并行组织学检查。结果Ⅰ、Ⅱ、Ⅲ组的血管通畅率分别为 80 %、60 %和 0。Ⅰ、Ⅱ两组血管移植后均存在一定程度的排斥反应 ,两组未见明显不同。结论　深低温保存同种异体主动脉的抗原性并没有明显减弱 ,但由于深低温较好地保存了血管活性和内膜层 ,在低压力的下腔静脉具有一定的应用前景。Objective To explore the feasibility of cryopreserved aortic homograft in replacement of the inferior vena cava.Methods A 4 cm long segment of the inferior vena cava was replaced by one of the following grafts: cryopreserved aortic homograft (group I, n =5); fresh aortic homograft (group II, n =5); Dacron synthetic vascular prosthesis (group III, n =5).The cryopreserved aortic homografts were stored at -196 ℃ for more than two weeks before grafting.All grafts were retrieved at 8 weeks and assessed by patency rate and histologic observation.Results The patency rates at 8 weeks were 80% in group I,60% in group II and 0 in group III.The cryopreserved specimens before implantation retained morphologic integrity except slight loss of the endothelium.The histological changes seen in the cryopreserved homografts were similar to those of the fresh homografts,including the partial loss of endothelium,shrinkage of smooth muscle in the medium,infiltration of the inflammatory cells in the adventitia.Conclusion Cryopreservation process does not markedly reduce the antigenicityof aortic homograft.The well preserved viability and endothelium of the cryopreserved aortic homograft make it possible to be one of ideal venous grafts.

关 键 词：腔静脉移植 深低温保存 犬 主动脉 腔静脉重建 

分 类 号：R654.3[医药卫生—外科学]