飞天蜈蚣七对肝纤维化大鼠肝组织转化生长因子β_1蛋白表达的影响  

Effect and Mechanism of Aralia Chinensis L. in the Treatment of Hepatic Fibrosis in Rats

作　　者：崔大江[1] 聂丹丽[1] 郅敏[2] 毛水龙 

机构地区：[1]西安交通大学第二医院中医科,西安710004 [2]西安交通大学第二医院消化内科,西安710004 [3]西安神龙中医医院,西安710061

出　　处：《中国中西医结合消化杂志》2003年第3期136-137,138,共3页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：陕西省自然科学基金(No.2000SM44)

摘　　要：目的:探讨飞天蜈蚣七防治肝纤维化的作用机制。方法:CCl_4法复制肝纤维化模型,48只SD大鼠随机分为飞天蜈蚣七组(A组)、秋水仙碱组(B组)、模型组(C组)和正常对照组(D组),每组12只。各组分别在第22、43天时,检测血清透明质酸(HA)、层粘蛋白(LN)、Ⅲ型前胶原(PC-Ⅲ)含量;行肝组织转化生长因子-β_1(TGF-β_1)免疫组化染色,检测其蛋白表达、结果:第22天时,与D组、A组比较,C组血清HA、PC-Ⅲ升高,TGF-β_1阳性表达较强(P<0.05),LN升高(P>0.05);第43天时,与D组比较,C组血清HA、LN、PC-Ⅲ明显降低,肝组织TGF-β_1阳性表达明显减轻(P<0.05)。结论:飞天蜈蚣七可以减轻肝纤维化程度、抑制肝组织TGF-β_1蛋白的表达,飞天蜈蚣七可作为抗肝纤维化的一种有效药物。Objective: To investigate the effect and mechanism of Aralia Chinensis L. in the treatment of hepatic fibrosis in rats. Methods: Forty-eight SD rats were randomly divided into 4 groups ( n =12 each): Aralia Chinensis L. group (group A), colchicines group (group B), hepatic fibrosis model group (group C) and normal control group (group D). Forty per cent carbon tetrachloride (CC14) dissolved in peanut oil was injected subcutaneously into rats of group A, B and C twice a week and the rats of group A and B were simultaneously treated with Aralia Chinensis L. and colchicines respectively. HA, LN, PC-Ⅲ in serum were detected by radioimmunity method and the expression of TGF-β was determined by immunohistochemical staining. Results: At the end of the third week, HA and PC-Ⅲ in the serum of group C increased significantly and the expression of TGF-pl raised strongly compared with those of group A and D ( P <0. 05), while LN increased without statistic significance ( P >0. 05). At the end of the sixth week, HA, LN, PC- I and the expression of TGF-β1, in liver tissue of group C decreased greatly compared with those of group D ( P <0. 05). Conclusion: Aralia Chinensis L. could relieve hepatic fibrosis and lessen the level of TGF-β1 in liver tissue. The result indicated that Aralia Chinensis L. was an effective drug for hepatic fibrosis.

关 键 词：飞天娱蚣七 肝纤维化 大鼠 肝组织 转化生长因子β1 蛋白表达 秋水仙碱 Ⅲ型前胶原 层粘蛋白 血清 

分 类 号：R285.5[医药卫生—中药学] R259.752[医药卫生—中医学]