低分子量肝素的制备与纯化  

Preparation and Purification of Low Molecular Weight Heparin

作　　者：张万忠[1] 胡晓东[2] 刘宁[1] 梁兵[1] 李文秀[1] 

机构地区：[1]沈阳化工学院,辽宁沈阳110142 [2]沈阳第三橡胶厂军代表室,辽宁沈阳110025

出　　处：《沈阳化工学院学报》2003年第2期114-117,共4页Journal of Shenyang Institute of Chemical Technolgy

摘　　要：　介绍以猪肠粘膜肝素粗品为原料,采用亚硝酸控制解聚法制备分子量在4000～8000D的低分子量肝素.采用正交实验法讨论反应温度、时间,亚硝酸浓度,溶液pH值等条件对解聚反应的影响.测定产物的分子量,经SephacrylS 200HR凝胶色谱柱对解聚的低分子量肝素进行分离纯化.由红外光谱13Cnmr谱图对其结构作鉴定.以粗品肝素作原料制备低分子量肝素,成本较为低廉,工业化前景比较广阔.A method of preparation of low molecular weight heparin from crude hog mucosal heparin by depolymerization with nitrous acid was studied. The optimization of the experimental conditions, such as temperature, time, concentration of nitrous acid and pH have been investigated. The depolymerized heparin having molecular weight of 4 000～8 000 Dalton was purified with column of Sephacryl S200. Average molecular weight of low molecular weight heparin were determined. The structure of the low molecular weight heparin was identified by using IR and 13CNMR spectra. Experimental results showed that method of preparation of low molecular weight heparin was satisfactory. The depolymerized product has lower cost from crude, So the technology of preparation of low molecular weight heparin from crude has broader prospect.

关 键 词：低分子量肝素 亚硝酸解聚 分子量测定 凝胶色谱 

分 类 号：R972[医药卫生—药品]