病毒灭活对皮质骨生物力学特性的影响  

Effect of virus inactivation on the biomechanical properties of cortical bone

作　　者：雷军[1] 顾洁夫[1] 蔡林[1] 汪鹏[1] 

机构地区：[1]武汉大学中南医院,湖北武汉430071

出　　处：《中华医院感染学杂志》2003年第5期417-420,共4页Chinese Journal of Nosocomiology

基　　金：湖北省科委科研基金资助项目 (972 912 )

摘　　要：目的　探讨既能保证异体骨移植的安全性 ,又能最大限度地保留其生物力学特性的γ射线灭菌剂量。方法　取新鲜猪股骨干 ,去除骨膜、软组织及骨髓 ,从股骨干中间横行截成两段 ,其中一段不予处理 ,作为对照组骨环的来源 ,另一段用来模拟 HBV感染的异体骨 ,将装有 1m l HBV悬液的玻璃管加盖软木塞后放入骨段的髓腔中 ,两端用铅封闭 ,做成了 HBV感染的骨模型 ,行不同梯度剂量的γ射线辐照 ,然后取 HBV悬液行病毒标志物 (HBs Ag,HBV- DNA)的检测 ,了解病毒破坏程度 ;从对照骨段和处理骨段截取相邻骨环行 3点弯曲试验 ,测量弹性模量、最大载荷 ,了解γ射线辐照对骨生物力学特性的影响。结果　 >2 5 k Gy的γ射线能完全破坏 HBV-DNA,完全破坏 HBs Ag需要 5 0 k Gy;γ射线对骨环的最大载荷的影响有剂量依赖性 ,剂量越大 ,最大载荷下降越多 ,<2 5 k Gy的γ射线辐照引起的最大载荷下降无统计学意义 (P >0 .0 5 ) ,>30 k Gy的γ射线辐照使骨环的最大载荷显著下降 (P <0 .0 5 )。结论　γ射线辐照对骨生物力学特性的影响有剂量依赖性 ,2 5 KGy的剂量不影响骨的生物力学特性 ,且能灭活 HBV。OBJECTIVE To investigate the proper dose of gamma irradiation to ensure the safety of banked bone and retain its biomechanical properties as much as possible. METHODS A fresh femoral diaphysis was cut into two sections from the middle. One section was assigned to control group, the other section was used to simulate HBV infected bone allograft. A tube containing suspension of hepatitis B virus was placed within the medullary cavity of this section and the ends of the section were sealed with lead. Then the bone/virus model was deep frozen and exposed to 5 50 kGy cobalt 60 gamma irradiation respectively. The virus destruction was evaluated by HBsAg and HBV DNA. The two neighboring rings of a femoral diaphysis were cut from the treatment and control sections and underwent three point bending test to determine gradient of elastic region (W/D) and maximum load sustained (W max ). RESULTS Gamma irradiation of 25 kGy or more could destroy HBV DNA and 50 kGy was required to destroy HBsAg completely. The gradient of elastic region (W/D) didn′t decrease significantly after irradiation with 5 50 kGy (P>0.05). Changes in W max were dose related. W max decreased while the dose was increased. W max din′t decrease significantly after irradiation with less than 25 kGy (P>0.05). There was a significant reduction in W max of the samples receiving 30 kGy and 50 kGy irradiation (P<0.05). CONCLUSIONS A dose of 25 kGy could perfectly inactivate HBV and had no effect on the biomechanical properties of cortical bone.

关 键 词：皮质骨 生物力学 乙型肝炎病毒 Γ射线 病毒灭活 

分 类 号：R658.1[医药卫生—外科学]