基因枪法向甘蓝型油菜转移反义FAD2基因的研究  

On Transferring Antisense FAD2 Gene into Brassica napus via Particle Bombardment

作　　者：熊兴华[1] 官春云[1] 李栒[1] 江巨鳌[1] 邬克彬[1] 王学军[1] 周小云[1] 

机构地区：[1]作物基因工程湖南省重点实验室,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2003年第3期188-191,共4页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南省科技厅资助项目(97JKY1005)

摘　　要：为了获得高油酸油菜品种,以湘油15号子叶柄为受体材料,用基因枪法,将反义油酸脱饱和酶FAD2基因连在种子特异表达载体上后导入油菜,使用可裂膜压力7700kPa,样品室真空度9.5×104Pa,质粒质量浓度为1.0μg/μL,靶距9cm,通过对子叶柄的预处理48h,高渗处理1h,获得8株再生植株.经PCR与PCR-Southern杂交检测,其中有2株为转化株,证明反义FAD2基因已整合到湘油15号基因组中.In order to raise the content of oleate in rapeseed,the trans desaturase gene(FAD2 gene)is introduced into Brassica napus by a particle bombardment method,using the cotyledon petioles of Brassica napus used as acceptor materials. 8 regenrated seedlings resistence to kan are obtained by using following parameters:rupture disk pressure 7 700 kPa,vaccum 9.5×104 Pa,target distance 9 cm, plasimid conc.1.0 μg/μL, cotyledon petioles preculture 48 h,and highosmotic media treatment 3 h.Detection of PCR and PCRSouthern indicates that the antisense FAD2 Gene is integrated into the DNA genome of rapeseed.

关 键 词：油酸脱饱和酶基因 转化 油菜 基因枪 

分 类 号：Q785[生物学—分子生物学]