伪狂犬病病毒弱毒株LY株的分离鉴定  

Isolation and Identification of an Attenuated Strain of Pseudorabies Virus

作　　者：马凤龙[1] 王文成[2] 李尚波[2] 马振宇[2] 丁建民[2] 卫广森 王铁东[4] 宣华[4] 

机构地区：[1]济南军区军事医学研究所,山东济南250014 [2]辽宁省益康生物制品厂,辽宁辽阳111000 [3]辽宁省动物防疫站,辽宁沈阳110161 [4]解放军军需大学,吉林长春130062

出　　处：《兽医大学学报》2003年第3期252-254,共3页

摘　　要：从辽阳某猪场的 1 0日龄仔猪中分离到 1株病毒 ,经纯化后测得其毒价为 1 0 7.2 9TCID50 / m L。细胞中和试验表明 ,该病毒能被猪伪狂犬病病毒标准阳性血清所中和。电镜下可见到典型的疱疹病毒粒子 ,具有囊膜及外周纤突。所分离的病毒对氯仿、胰蛋白酶、乙醚敏感 ,在 p H5 .0～ 9.0下稳定 ,5 6℃ 30 m in可以灭活。应用特异性引物 ,通过 PCR能扩增出伪狂犬病病毒 1 2 4 0 bp的 g D基因。分离病毒对 3日龄乳鼠有一定的致病力 ,但对家兔、3～ 5日龄仔猪及妊娠母猪都有很高的安全性。用不同剂量的病毒培养液肌肉注射于 3～ 5日龄仔猪 ,1 4 d后用 1 0 5.7TCID50 伪狂犬病病毒强毒攻击 ,所有试验仔猪均可得到有效保护。用分离毒免疫母猪 ,其后代可获高滴度的母源抗体 ,1 5日龄的仔猪能抵抗1 0 5.7TCID50 强毒的攻击。试验的结果初步说明 ,所分离的病毒为伪狂犬病病毒 (命名为 PRV L Y株 ) ,并可能是一株弱毒株 。A strain pseudorabies virus named LY strain was isolated from pigs in Liaoyang.The virus isolate was purified by plaque technique on CEF monolayer with the titer of 10 7 29 TCID 50 /mL.Virus neutralization(VN) test showed that the isolate could be neutralized by PRV positive serum.Envelope and peplomers were observed on the surface of the virus under electron microscope.With the special primer,the gD gene about 1 240 bp was obtained by PCR.The virus isolate was pathogenic to 3 days mice and safe to rabbits and neonatal pigs.The neonatal pigs inoculated with different doses (10 0,10 -1 ,10 -2 mL) of the isolate showed no clinical signs when challenged with virulents train of PRV after a 14 day vaccination.Weaned pigs acquired high titer maternal antibodies from vaccinated sows and could be protected against PRV infection.Antibody to the isolate was detected from the vaccinated pigs.According to the above results,it was suggested that the virus isolate might be an attenuated PRV virus with good protection against virulent strain of PRV.

关 键 词：伪狂犬病病毒 弱毒株LY株 分离 鉴定 

分 类 号：S852.65[农业科学—基础兽医学]