CTLA4Ig重组腺病毒载体的构建与体外表达  

Construction and identification of recombinant adenovirus vector containing CTLA4Ig gene

作　　者：郭小荑[1] 邓宇斌[1] 陆才生[2] 李树浓[1] 

机构地区：[1]中山大学中山医学院病理生理学教研室,广东广州510080 [2]中山大学附属第三医院风湿科,广东广州510630

出　　处：《中国病理生理杂志》2003年第5期585-589,T002,共6页Chinese Journal of Pathophysiology

基　　金：广东省自然科学基金资助项目 (No.A10 16 89);中法合作基金资助项目 (No.PRAB0 1- 0 7);广东省卫生厅基金资助项目 (No .4 2 12 0 6 4);广东省科技计划项目(2 0 0 2C5 0 5 0 10 4 )

摘　　要：目的 :构建含有CTLA4Ig基因的重组腺病毒载体 ,为下一步在动物模型体内表达和基因治疗提供基础。方法 :自行设计一对分别含有BglⅡ及HindⅢ酶切位点的CTLA4Ig基因上下游引物 ,以质粒PCDNA3 0 /CTLA4Ig为模板 ,通过PCR扩增获得CTLA4Ig基因全部序列。片段回收以后经BglⅡ及HindⅢ双酶切 ,定向插入到腺病毒穿梭质粒pAdTrack -CMV的启动子下游BglⅡ及HindⅢ位点之间 ,获得重组质粒pAdTrack -CTLA4Ig。通过BglⅡ /HindⅢ双酶切、PCR及插入片段测序鉴定后 ,将正确重组体pAdTrack -CTLA4Ig与腺病毒骨架质粒pAdEasy - 1共转化BJ5 183细菌。同源重组后用选择性培养基筛选阳性克隆 ,提取质粒用脂质体介导转染 2 93细胞 ,通过观察绿色荧光蛋白(GFP)的表达及PCR扩增目的基因等方法鉴定重组的腺病毒。结果 :成功构建了含有CTLA4Ig基因的重组腺病毒 ,病毒滴度为 1 6 5× 10 12 PFU/L。结论 :该重组腺病毒的构建为下一步研究其在哺乳动物细胞内表达、研究CTLA4Ig的生物学活性及移植排斥、自身免疫病的基因治疗提供了一定的基础。AIM: To construct a recombinant adenovirus expression vector containing CTLA4Ig gene.METHODS: The CTLA4Ig gene derived from the plasmid PCDNA3.0/CTLA4Ig by using polymerase chain reaction (PCR) was inserted into the backward position of cytomegalovirus (CMV) immediate early promoter of the shuttle plasmid (pAdTrack-CMV). After being identified by endonuclease, PCR and sequencing, the recombinant shuttle plasmid pAdTrack-CTLA4Ig was co-transformed into E.coli. BJ5183 cells with the adeoviral backbone plasmid pAdEasyl-1 to obtain the homologous recombination. The adenovirus was generated in 293 cells. A series methods such as PCR and fluorescence microscope was employed to identify the generated recombinant adenovirus. RESULTS: Recombinant CTLA4Ig adenoviruses were constructed and the titer of virus was generally up to 1.65×10 12 phaque forming units per liter (PFU/L). CONCLUSION: Success in constructing recombinant pAdTrack-CTLA4Ig will be the base of the further research on its expression in the mammalian cells, and be potenially used in the prevention of transplant rejection and autoimmunity diseases.

关 键 词：CTLA4Ig重组腺病毒载体 构建 体外表达 T淋巴细胞 细胞塞件 免疫球蛋白 基因治疗 移植排斥 

分 类 号：R392.4[医药卫生—免疫学] R394[医药卫生—基础医学]