共有序列寡核苷酸竞争结合NF-κB以减少TNF产生的实验研究  

The consensus oligonucleotide competitive binding with NF-κB inhibits TNF production

作　　者：陈宁[1] 叶静[1] 汤特[1] 朱学良[1] 

机构地区：[1]天津医科大学病理生理学教研室,天津300070

出　　处：《中国病理生理杂志》2003年第5期619-621,共3页Chinese Journal of Pathophysiology

基　　金：天津医科大学科研基金资助项目 (No .99KY2 )

摘　　要：目的 :了解使用含核因子 -κB(NF -κB)结合位点序列的共有序列寡核苷酸在胞浆内与NF -κB结合 ,以减少LPS刺激TNF产生的效果。方法 :直接将经硫代磷酸修饰的寡核苷酸导入大鼠腹腔巨噬细胞 ,以减少细胞分泌产生肿瘤坏死因子 (TNF)的效果作为判断指标 ,以ELISA法检测细胞培养上清液中的TNF -α含量。结果 :加入具有NF -κB结合位点序列的共有序列寡核苷酸 ,可明显减少巨噬细胞受LPS刺激后所产生的TNF -α。结论 :结果提示 ,具有NF -κB结合位点的共有序列寡核苷酸的使用 ,可在内毒素脂多糖 (LPS)致病过程的中心环节上阻止或减轻LPS诱导的TNFAIM: To investigate whether a consensus oligonucleotide, which contains nuclear factor-kappa B(NF-κB) binding site, decreases TNF production stimulated by lipopolysaccharide(LPS). METHODS: The thiophosphoric acid modified oligonucleotides were transferred into rat peritoneal macrophages directly, TNF content in LPS-stimulated cell culture supernatant was determined by ELISA.RESULTS: Adding the consensus oligonucleotides with NF-κB binding site into macrophages markedly decreased TNF production following LPS stimulation. CONCLUSION: These results show that use of the consensus oligonucleotides with NF-κB binding site, which can combine with NF-κB, can block or decrease TNF production induced by LPS in macrophages.

关 键 词：多器官功能衰竭 肿瘤坏死因子 核因子—κB NF—κB 寡核苷酸 实验研究 

分 类 号：R59[医药卫生—内科学]