消渴颗粒剂对糖尿病肾病大鼠血糖、血脂含量的影响  

Influence of Xiaoke keli Ji on glycemia and serum lipoprotein levels in diabetic nephropathy rats

作　　者：王谦[1] 龚慕辛[2] 赵辉[2] 王跃秀[2] 于玫[2] 李克玲[1] 黄启福[1] 李伯光[1] 严京[1] 

机构地区：[1]北京中医药大学病理学教研室,北京100029 [2]首都医科大学中医药学院,北京100013

出　　处：《中国病理生理杂志》2003年第5期664-667,T004,共5页Chinese Journal of Pathophysiology

基　　金：北京市教育委员会科技发展计划项目 (No .0 0KJ- 0 74 )

摘　　要：目的 :观察糖尿病肾病大鼠血糖、胆固醇、甘油三脂含量的变化以及消渴颗粒剂对其的影响。方法 :采用 3/ 4肾切除 ,腹腔一次性注射STZ复制大鼠糖尿病肾病模型。将动物分为模型组、消渴颗粒剂治疗组、阳性药对照组和假手术组。每组大鼠于注射STZ后 1、2、3、4、5周尾尖取血测定空腹血糖、血清肌酐、血清胆固醇和血清甘油三脂含量。各组大鼠于注射STZ后 6周杀检 ,进行肾脏形态学观察。结果 :注射STZ后 6周 ,模型组大鼠有不同程度的肾小球硬化。中药治疗组上述病变明显轻于病理组。注射STZ后第 1、2、3、4、5周 ,模型组血清肌酐明显高于假手术组 (P <0 0 5 ) ,治疗组则明显低于模型组和阳性对照组。注射STZ后 1、2、3、4、5周模型组空腹血糖、血清胆固醇明显高于假手术组 (P <0 0 5 ) ,血清甘油三脂在注射STZ后 1、2、3、5周明显高于假手术组 (P <0 0 5 ) ;消渴颗粒剂治疗组和阳性对照药组与模型组同期比较病变明显减轻。空腹血糖和血清胆固醇、甘油三脂含量呈正相关。结论 :采用此方法成功地复制了大鼠糖尿病肾病模型 ,糖尿病肾病大鼠血糖增高可能引起血脂增高 ,消渴颗粒剂治疗糖尿病肾病的途径之一是降低大鼠血糖、血清胆固醇、血清甘油三脂含量 。AIM: To observe the changes of glycemia and serum cholesterol and triglyceride in diabetic nephropathy rats and therapeutic effects of Xiaoke Keli Ji. METHODS: 3/4 nephrectomy was adopted firstly, three weeks later streptozotocin(STZ) was administered intraperitoneally to establish diabetic nephropathy model in rats. Animals were divided into four groups:model group, Xiaoke Keli Ji treatment group, positive control group and sham group. Changes of serum sugar and serum creatinine, cholesterol and triglyceride were examined at 1, 2, 3, 4, 5 weeks after STZ injection. Renal tissue samples were adopted at 6th week and studied by light microscopy. RESULTS: Model group demonstrated different degree of glomerular sclerosis. Lesions in treatment group were alleviated. Serum creatinine, serum sugar and serum cholesterol were higher at 1, 2, 3, 4, 5 weeks after STZ injection in model group than that of the sham group ( P< 0. 05), serum triglyceride was higher at 1, 2, 3, 5 weeks after STZ injection in model group than that of the sham group( P< 0. 05). Xiaoke Keli Ji reduced those changes. Serum sugar was in positive correlation with serum lipoprotein. CONCLUSION: Diabetic nephropathy model was duplicated successfully. High serum sugar may lead to high serum lipoprotein, Xiaoke Keli Ji may treat diabetic nephropathy by reducing serum sugar and serum cholesterol and triglyceride.

关 键 词：糖尿病肾病 大鼠 血糖 血脂 胆固醇 甘油三脂 消渴颗粒剂 中医药疗法 

分 类 号：R259.872[医药卫生—中西医结合] R286.715[医药卫生—中医内科学]