人腹主动脉瘤平滑肌细胞表型变化  

Phenotypic changes of vascular smooth muscle cells in human abdominal aortic aneurysms

作　　者：吴建秋[1] 景在平[1] 

机构地区：[1]第二军医大学附属长海医院血管外科,上海200433

出　　处：《中国病理生理杂志》2003年第5期595-598,T002,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No .39770 72 1)

摘　　要：目的 :研究腹主动脉瘤 (AAA)中血管平滑肌细胞 (VSMC)表型改变 ,探讨其在AAA发病中的作用。方法 :选取人体AAA、动脉闭塞性疾病 (AOD)和正常腹主动脉 (NA)组织 ,采用α -肌动蛋白 (α -SMA)、结蛋白(desmin)及肌球蛋白重链 3种表型 (SM1、SM2和SMemb)单克隆抗体 ,利用免疫组化及电镜技术 ,检测VSMC收缩型与合成型。结果 :AOD及NA中VSMC以收缩表型α -SMA、desmin、SM1和SM2表达为主 ,SMemb在AOD的表达较低 ,而在NA无表达。AAA中VSMC以合成表型SMemb为主 ,α -SMA、SM1和SM2明显低于AOD及NA ,desmin不表达 ;其中破裂者的SMemb和SM2低于非破裂者。结论 :VSMC表型变化参与腹主动脉壁损伤重构 。AIM:To study phenotypic changes of vascular smooth muscle cells (VSMC) in abdominal aortic aneurysmal (AAA) pathogenesis. METHODS: Tissue samples of human infrarenal aneurysmal and normal aorta(NA), and arterial occlusive diseases(AOD) were evaluated. Monoclonic antibodies of α-smooth muscle actin(α-SMA), desmin and smooth muscle myosin heavy chain isoforms (SM1, SM2 and SMemb) were used in immunohistochemistry to determine VSMC isoforms. Immunohistochemical results were analyzed with the use of computer-generated image technique. Ultrstructures of VSMC in three tissues above were observed by electron microscope. RESULTS: In control AOD and NA, VSMC in the media were strongly immunostained for α-SMA, desmin, SM1 and SM2. Immunoreactivity for SMemb was faint or weakly positive in AOD, but negative in NA. In AAA,the balance shifts to SMemb predominance with suppressed α-SMA, SM1 and SM2 and negative desmin, while in ruptured aneurysmal walls, the expression of SM2 and SMemb were decreased compared with the non-ruptured aneurysmal walls. CONCLUSION:Phenotypic changes of VSMC are concerned with abdominal aortic structure lesion and remodeling, which contributes to AAA formation and development.

关 键 词：腹主动脉瘤 平滑肌细胞表型 超微结构 肌球蛋白 肌动蛋白 结蛋白 免疫组化 

分 类 号：R543.16[医药卫生—心血管疾病]