人参二醇组皂甙对家兔缺血再灌注损伤心肌线粒体的影响  

EFFEC OF PANAXADIOL SAPONINS ON MITOCHONDRIAES OF RABBIT'S ISCHEMIC AND REPERFUSED MYOCARDIUM

作　　者：陈立波[1] 崔东哲[1] 刘玉梅[2] 林柏松[2] 

机构地区：[1]北京大学深圳医院,深圳518036 [2]吉林大学中日联谊医院,长春130031

出　　处：《中国中医药科技》2003年第3期152-153,共2页Chinese Journal of Traditional Medical Science and Technology

基　　金：吉林省中医药管理局重点课题;吉中药科2000-L17

摘　　要：目的:研究人参二醇组皂甙(Panaxadiol Saponins,PDS)对缺血再灌注损伤心肌超微结构线粒体的影响。方法:应用离体作功兔心模型,将不同剂量的PDS加入St.ThomasⅡ号心停搏液中,电镜下观察心肌缺血再灌注前后心肌超微结构线粒体的变化,以Flameng线粒体半定量法评价记分。结果:在40～160mg/L的浓度范围内,PDS具有减轻缺血再灌注后心肌线粒体损伤的作用。在其浓度增至320mg/L时,上述作用消失,反有加重心肌线粒体损伤的倾向。结论:PDS有抗心肌缺血再灌注损伤作用,该作用有严格的浓度-效应关系。Objective: To study the effect of panaxadiol saponins ( PDS) on mitochondriaes of the ischemic and reperfused myocardium in the rabbit. Methods:The different dosage of PDS were treated as an additive agent to cardioplegic solution on ischemic and reperfused myocardium using isolated working rabbit heart.The samples of the myocardial biopsy were observed and mitochondria were scored by Flameng' g method under the electron microscope. Results: As an additive agent to cardioplegic solution, the dosage of PDS in the range of 40 - 160mg/L can reduce the injury of mitochondriaes on ischemic and reperfused myocardium, when the concentration of PDS was increased to 320mg/L,the above effects were lost, the mitochondria injury were aggravated. Conclusion: PDS can effectively prevent the ischemic and reperfused myocardium injury, the effect is closely relative to the medical concentration.

关 键 词：人参二醇组皂甙 家兔 心肌缺血 再灌注损伤 心肌线粒体损伤 人参总皂甙 保护作用 

分 类 号：R285.5[医药卫生—中药学] R259.422[医药卫生—中医学]