胰岛素受体基因多态性与高血压肾损害相关性研究  

Relation between polymorphism of insulin receptor gene exon 8 and hypertensive nephrosclerosis

作　　者：徐岩[1] 崔岩[1] 马瑞霞[1] 崔莉[1] 张乐[1] 

机构地区：[1]青岛大学医学院附属医院,山东青岛266003

出　　处：《山东医药》2003年第13期4-6,共3页Shandong Medical Journal

基　　金：青岛市科委资助课题 (No 2 0 0 1KNS 2E 48 2 )

摘　　要：为探讨原发性高血压 (EH)肾损害与胰岛素受体 (INSR)基因第 8外显子NsiⅠ酶切多态性的关系 ,采用生化法、多聚酶链反应 (PCR)和限制性内切酶片段长度多态性分析 (RFLP)等技术 ,检测了N2 等位基因在EH肾功能正常 (EH NRF)组、EH肾损害尿毒症 (EH CRF)组及正常对照 (NC)组中的表达。结果显示 ,NsiⅠ多态性与患者的年龄、体重指数 (BMI)、总胆固醇 (TC)及甘油三脂 (TG)等无相关性 ;N2 等位基因频率在EH NRF组及EH CRF组均明显高于NC组 (P <0 0 5、<0 0 1) ,且在EH CRF组高于EH NRF组。提示N2 等位基因可能是中国人EH(特别是EH CRF)To investigate the relation between polymorphism of insulin receptor (INSR) gene exon 8 and hypertensive nephrosclerosis,polymerase chain relation (PCR) and restriction fragment length polymorphism (RFLP) combined with biochemistry method were used to measure the frequency of N 2 allele of INSR gene in normal renal function of essential hypertension (EH-NRF),renal failure of hypertensive nephrosclerosis (EH-CRF) and normal control (NC) subjects.Results showed that there was no relationship between polymorphism of NsiⅠ and the age,body mass index (BMI),serum lipid level.The N 2 allele frequency of INSR gene in EH-NRF and EH-CRF groups were higher than that in NC group(P<0.05,<0.01),and the N 2 allele frequency of INSR gene in EH-CRF group was also higher than that in EH-NRF group.These suggest N 2 allele is a significantly sensitive gene of Chinese EH and EH-CRF.

关 键 词：胰岛素受体 基因多态性 高血压肾损害 原发性高血压 生化法 多聚酶链反应 

分 类 号：R544.1[医药卫生—心血管疾病] R692[医药卫生—内科学]