新型含磷抗原的合成  

Synthesis of a Novel Antigen Containing P

作　　者：谢桂阳[1] 陈柳民[1] 陈家华[1] 金声[1] 

机构地区：[1]北京大学化学与分子工程学院化学生物学系,生物有机和分子工程教育部重点实验室,北京100871

出　　处：《高等学校化学学报》2003年第6期1037-1039,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :2 9972 0 0 1)资助

摘　　要：It is of great importance to study the catalytic antibody that catalyzes the hydrolysis of peptide bond. The synthesis of a new kind of antigen(10) containing phosphorus was reported in this paper, which was designed and synthesized according to hippuryl phenylalanine, a substrate of carboxypeptidase A(CPA). To mimic the structure of the substrate, the free C-terminal carboxylic group was remained. However, it was not easy to synthesize the phosphonamide peptide hapten(7) directly, so compound 5 was synthesized firstly, after the catalytic hydrogenolysis it was coupled with p-nitrobenzoyl chloride to get compound(7). The synthesis of antigen and further preparation of antibody will be a good foundation for the activity study of the antibody.It is of great importance to study the catalytic antibody that catalyzes the hydrolysis of peptide bond. The synthesis of a new kind of antigen(10) containing phosphorus was reported in this paper, which was designed and synthesized according to hippuryl phenylalanine, a substrate of carboxypeptidase A(CPA). To mimic the structure of the substrate, the free C-terminal carboxylic group was remained. However, it was not easy to synthesize the phosphonamide peptide hapten(7) directly, so compound 5 was synthesized firstly, after the catalytic hydrogenolysis it was coupled with p-nitrobenzoyl chloride to get compound(7). The synthesis of antigen and further preparation of antibody will be a good foundation for the activity study of the antibody.

关 键 词：含磷抗原 合成 肽键 水解 抗体酶 酰胺键 过渡态结构 

分 类 号：Q553[生物学—生物化学] R392.11[医药卫生—免疫学]