人肾小管上皮细胞的原代培养及鉴定  

Primary Culture and Identification of Human Renal Tubular Epithelia Cells

作　　者：涂明利[1] 陈双郧[2] 王卫民[3] 陈霞萍[3] 曹树军[2] 

机构地区：[1]郧阳医学院附属太和医院呼吸内科,湖北十堰442000 [2]郧阳医学院附属太和医院妇产科,湖北十堰442000 [3]郧阳医学院附属太和医院生命科学研究所,湖北十堰442000

出　　处：《郧阳医学院学报》2002年第6期330-332,共3页Journal of Yunyang Medical College

摘　　要：目的 :建立人肾小管上皮细胞原代培养、传代、冻存及鉴定方法。方法 :采用肾小管节段贴块培养法 (A法 )与消化培养法 (B法 )对比原代培养 ,并以 0 .2 5 %胰蛋白酶消化、传代 ,以免疫细胞化学方法鉴定细胞种类 ,常规冻存和复苏。结果 :A法成功培养、传代、冻存、复苏并鉴定了人肾小管上皮细胞 ,B法失败。结论Objective To establish a method for primary culture, passage and identification of human renal tubular epithelial cells. Methods Grinding-centrifugalizing (Group A) and grinding digesting (Group B) were respectively used in primary culture, and 0.25% trypsase were used in passage culture. The cell types were identified by immunocytochemistry. Results The primary and secondary culture of human renal tubular epithelial cells of Group A were both successful, so was the identification of the cultured cells. The primary culture of human renal tubular epithelial cells of Group B failed to be finished. Conclusion The present method (Group A) is an efficient means for the primary culture, passage and identification of human kidney tubular epithelial cells. \[

关 键 词：人肾小管上皮细胞 原代培养 鉴定 兔疫细胞化学 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]