北京地区汉族妇女雌激素受体基因PvuⅡ多态性与骨密度的关系  

PvuⅡ polymorphisms of the estrogen receptor gene and bone mineral density in Beijing Han women

作　　者：薛延[1] 李东 王芊[1] 董兆文 

机构地区：[1]北京积水潭医院生化研究室,100035 [2]国家计生委科学技术研究所分子生物研究室

出　　处：《北京医学》2003年第1期6-9,共4页Beijing Medical Journal

基　　金：国家自然科学基金资助项目 (3 9970 660 )

摘　　要：目的　探讨北京地区汉族妇女雌激素受体 (ER)基因PvuⅡ多态性与骨密度 (BMD)的关系。方法　采用聚合酶链反应 限制性片段长度多态性 (PCR RFLP)法 ,检测 179例北京地区汉族妇女ER基因PvuⅡ多态性。采用双能X线吸收仪 (DEAE)测量腰椎、近端股骨及前臂骨密度 ,并分析其相关性。结果　北京地区汉族妇女ER基因 pp、Pp、PP基因型频率分别为 0 .35 2、0 .4 6 4和 0 .184 ,绝经前与绝经后妇女无显著性差异 ,绝经后妇女腰椎骨密度为 0 .836± 0 .180 g/cm2 ,明显低于未绝经妇女的 1.0 38± 0 .14 0 g/cm2 ,绝经后妇女骨质疏松发病率为 5 4 .3%。结论　ER基因PvuⅡ基因型频率分布有种族差异。ER基因PvuⅡ基因型与骨密度无明显相关性。Objective To study the relationship between PvuⅡ polymrphisms of the receptor (ER) gene and bone mineral density in Beijing Han women.Methods Bone mineral density (BMD) was measured at lumbar spine,proximal femoral and arm by dual energy X ray absorptionetry (DEXA) and ER gene was determined by polymerase chain reaction restriction fragment length polymorphism (PCR RFLP) in 179 Beijing Han Women.Results The frequency distribution of the ER genotype of pp Pp PP respectively was 0.352,0.464 and 0.184 in Beijing Han women.There was no difference between premenopausal and postmenopausal women in frequency of the PvuⅡ genotype.The BMD of lumbar spine in postmenopausal women(0.836±0.180g/cm 2) was significant lower than that in premenopausal women (1.083± 0.140 g/cm 2, P <0.01) and the incidence of osteoporosis in postemenopausal women was 54.3%.Conclusions Frequency of PvuⅡ genotype of ER gene in Han women was appparently different from that in other races.The PvuⅡ genotype of ER gene was not associated with BMD in Beijing Han women.

关 键 词：雌激素受体 PvuⅡ基因 基因多态性 骨密度 聚合酶链反应-限制性片段长度多态性法 双能X线吸收仪 

分 类 号：R346[医药卫生—基础医学]