扩张型心肌病患者红细胞免疫功能、超氧化物歧化酶及血浆过氧化脂质的检测及临床意义  

Detection of erythrocytic immune function,superoxide dismutase and plasma lipoperoxide in patients with dilated cardiomyopathy and their clinical significance

作　　者：陈灿[1] 邵义明[1] 许金成[1] 李沛[2] 

机构地区：[1]广东医学院附属医院心血管内科,广东湛江524001 [2]广东医学院预防医学教研室,广东湛江524001

出　　处：《广东医学院学报》2002年第2期93-94,共2页Journal of Guangdong Medical College

摘　　要：目的 :探讨扩张型心肌病 (DCM)患者红细胞免疫功能与过氧化脂质的关系。方法 :选取本院 DCM患者共 1 1 8例 ,按 NYHA标准分组 ,心功能 级组 41例、 级组 37例、 级组 40例 ;健康人对照组 48例。两组同步检测红细胞 C3b受体、血浆丙二醛 (MDA)及红细胞超氧化物岐化酶 (SOD)水平 ,其中 ,红细胞 C3b受体检测采用红细胞酵母菌混合花环法 ,MDA采用硫代巴比妥酸 (TBA)法测定 ,红细胞 SOD测定采用邻苯三酚自氧化反应法 ,并作比较和相关分析。结果 :DCM心功能 级患者红细胞受体花环率 (RBC- C3b RR)和红细胞免疫复合物花环率 (RBC- ICR)较心功能 级、 级患者及对照组均明显降低 ,差异具显著意义 (P<0 .0 1 ,<0 .0 5)。相关分析表明 ,RBC- C3b RR与 MDA呈高度负相关、与 SOD呈中度正相关 ,相关系数分别为 - 0 .83和 +0 .72。结论 :DCM患者存在红细胞免疫功能下降及 LPO增加 ,并随心功能的减弱而加重 。Objective:To investigate the relationship between erythrocytic immune function and plasma lipoperoxide in patients with dilated cardiomyopathy (DCM) Methods: The level of erythrocytic C 3b receptor,plasma malonyl dialdehyde(MDA) and erythrocytic superoxide dismutase(SOD) was determined by red cell yeast mixture rose method,thiobarbituric acid method and pyrogallol self oxidizing method respectively,in 118 patients with DCM and 48 healthy persons The patients were divided into grade Ⅱ ( n =41),grade Ⅲ ( n =37) and grade Ⅳ ( n =40) according to NYHA classification standard of cardiac function Results: The level of red blood cell C 3b receptor rosette (RBC C 3b RR)and red blood cell immune complex rosette (RBC ICR) in patients with grade Ⅳ was noticeably lower than that in those with grade Ⅱ,grade Ⅲ and controls ( P <0 05 0 01) There were a negative correlation between RBC C 3b RR and MDA ( r =-0 83, P <0 01)and a positive one between RBC C 3b RR and SOD( r =0 72, P <0 01) Conclusion: It could be related to lipid peroxidation injury that down regulation of erythrocytic immune function and up regulation of LPO vary with deterioration of cardiac function

关 键 词：扩张型心肌病 红细胞 免疫功能 超氧化物歧化酶 血浆 过氧化脂质 检测 

分 类 号：R542.2[医药卫生—心血管疾病]