二巯基敌枯双生物作用的实验研究Ⅱ:组织化学观察  

EXPERIMENTAL STUDY ON BIOLOGICAL EFFECTS INDUCED BY N',N-METHYLENE-BIS(2-AMINO-5-SULFHYDRYL-1,3,4-THIADIANOLE)Ⅱ:OBSERVATION ABOUT HISTOCHEMISTRY

作　　者：程薇波[1] 孙丁[2] 李群英[1] 吴德生[2] 

机构地区：[1]四川大学华西公共卫生学院毒理教研室,610041 [2]四川大学华西公共卫生学院环境卫生教研室

出　　处：《现代预防医学》2003年第3期309-311,共3页Modern Preventive Medicine

基　　金：国家自然科学基金重点课题 (批准号 :3 0 0 3 0 12 0 );纽约中华医学基金资助

摘　　要：目的 :研究甲状腺激素干扰物二巯基敌枯双所致甲状腺组织化学的改变 ,并探讨所致甲状腺增生的最佳取材时间和敏感指标。方法 :Wistar大鼠 6 0只 ,随机分成正常对照组和实验组 ,每组各 30只。正常对照组给予溶剂二甲基亚砜 ,实验组给予二巯基敌枯双的二甲基亚砜溶液 ,剂量为 5 0 m g/ kg体重。于实验组的给药第 5 d、第 10 d和第 2 0 d,分别放血处死两组动物。用酶组织化学方法观察甲状腺琥珀酸脱氢酶 (SDH)、甲状腺过氧化酶酶活性的变化 (TPO) ;用免疫酶法测定核增殖抗原 (PCNA)在甲状腺中的阳性表达。结果 :实验组各期甲状腺滤泡上皮内 SDH和 TPO酶活性明显增高 (P<0 .0 5 ) ,实验组的各期 PCNA表达阳性细胞数也明显高于同期对照组 (P<0 .0 5 )。结论 :甲状腺激素干扰物二巯基敌枯双所致甲状腺滤泡上皮内 SDH和 TPO酶活性增高是 TSH刺激的结果 ,其最佳取材时间应是 5 d以前 ,而敏感指标应该是核增殖抗原 (PC-NA)。Objective:To study histochemistry changes of thyroid induced by N',N-methylene-bis (2-amino-5-sulfhydryl-1,3,4-thiadianole),and discuss appropriare time of exposure and sensitive index.Methods: Sixty Wistar rats were divided into 2 group including experiment and control group.Experiment group were fed with this agent for 50mg/kg bw.They were sacrificed by bleeding from femoral artery at fifth,10th,20th day,respectively,Enzyme activity of succinate dehydrogenase and thyroidperoxidase were observed by enzyme histochemistry and positive expression of PCNA in thyroid gland were examined by immunity histochemistry. Results:The results showed that activity of SDH and TPO in thyroid cell increased significently in experiment group( P< 0.05),while positive expression of PCNA in thyroid gland increased significantly in experiment group as well ( P <0.05).Conclusion:Increase of enzyme activity about succinate dehydrogenase and thyroidperoxidase induced by thyroid hormone disruptors N',N-methylene-bis (2-amino-5-sulfhydryl-1,3,4-thiadianole) were attributed to thyroid compensatory,Appropriare time of exposure was before 5 day and sensitive index was PCNA.

关 键 词：二巯基敌枯双 生物作用 实验 组织化学 甲状腺增生 甲状腺激素干扰物 

分 类 号：R114[医药卫生—卫生毒理学]