以萘酚绿B为介体的葡萄糖生物传感器  

Glucose Biosensor with Naphthol Green B as Mediator

作　　者：莫昌莉[1] 赵勤[1] 柴雅琴[1] 刘颜[1] 袁若[1] 

机构地区：[1]西南师范大学化学化工学院

出　　处：《化学传感器》2003年第1期26-31,共6页Chemical Sensors

基　　金：教育部优秀青年教师资助计划;重庆市科委自然科学基金

摘　　要：该文以聚乙烯醇缩丁醛为固定葡萄糖氧化酶(GOD)的载体,将GOD附在铂丝电极上制得葡萄糖酶电极。文中讨论了溶解性媒介体萘酚绿B的浓度、溶液的pH值和温度对该电极电流响应的影响。这一介体型葡萄糖传感器在优化的实验条件下,对葡萄糖表现出良好的响应特性,如响应快、重现性和稳定性好。传感器线性范围为8.0×10^(-4)mol/L-3.6×10^(-2)mol/L,相关系数为0.9933,检测限为5.0×10^(-4)mol/L。A new glucose biosensor was made of a platinum electrode modified by glucucose oxidase in polyvinyl butyral membrane. Electrocatalytic oxidation of glucose at the sensor was determinated with respect to solution pH, temperature and concentration of water - soluble mediator naphthol green B. Under optimal conditions, the sensor displayed a good linear response in the concentration range of 8. 0×10 -4 mol/L -3. 6 × 10 -2 mol/L, and the detection limit is 5. 0 × 10 -4 mol/L. Besides, the biosensor had fast response, excellent reproducibility and stability.

关 键 词：生物传感器 葡萄糖氧化酶 萘酚绿B 循环伏安法 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]