全身放射损伤对皮肤伤口组织细胞的影响及康复新的促愈作用(英文)  

The effect of radiation injury on histiocyte of skin wound and the repaired- promoting action of Kangfuxin

作　　者：舒崇湘[1] 冉新泽[1] 程天民[1] 阎国和[1] 

机构地区：[1]解放军第三军医大学预防医学系全军复合伤研究所,重庆市400038

出　　处：《中国临床康复》2003年第11期1644-1646,共3页Chinese Journal of Clinical Rehabilitation

基　　金：国家重点基础研究发展规划资助项目("973"项目)(G1999054205)~~

摘　　要：目的研究全身放射损伤对皮肤伤口组织细胞的影响及康复新(W11-α12)的促愈作用,探讨放创复合伤时创伤难愈的机制与促愈措施。方法100只雄性健康昆明种小鼠,体质量(20±2)g,以随机数字表法设单纯创伤(单伤)和全身放射损伤合并创伤(复合伤)组。小鼠6Gy照射后,于背部造成两个皮肤切口伤,观察伤后伤口愈合速度、伤口炎细胞、成纤维细胞和毛细血管数量及表皮细胞中碱性成纤维细胞生长因子(bFGF)含量的变化。结果伤后3,5,7d,单纯创伤组(单伤组)伤口愈合速度(%)为63.22,83.47,94.61,放创复合伤组(复合伤组)则显著降低(t=7.84,7.26,8.18,P<0.01),分别为52.67,69.18,82.88。伤后1,3,5d,复合伤组伤口炎细胞数量分别比单伤组下降26.53%,62.11%,38.91%;成纤维细胞数量下降43.73%,52.70%,25.32%;毛细血管数量在伤后5d降低55.97%;应用康复新后,两伤组均见伤口愈合速度加快、细胞数量增加,但复合伤组的增幅要大于单伤组。伤后3,5d,复合伤组表皮细胞bFGF含量比单伤组低59.15%和36.15%,当应用康复新后,复合伤组增幅分别比单伤组高35.90%和10.97%。结论伤后伤口局部细胞数量减少和表皮细胞中bFGF含量下降是合并放射损伤后创面难愈的重要原因之一,康复新对伤口的细胞游走、增殖及表皮细胞分泌bFGF的功能具有促进作用。Aim To investigate the effect of radiation injury of whole body on histiocyte in skin wound and Kangfuxin(W11 α12) and explore the mechanism of difficult recovery of combined wound and measures.Methods 100 male and healthy mice of Kunming series weighted (20±2) g.The experimental group of simple wound and radiation damaged combined with trauma was made respectively.The rats irradiation was accomplished with a dose of 6 Gy using a 60Co γ ray irradiation,then two incisions wound on back were made.To observe speed of wound healing and content changes inflammatory cells in wound,fibroblast,quantity of blood capillary and bFGF.Results On the 3rd,5th and 7th day after injury,the recovery speed(%) of wound in simple wound group was 63.22%,83.47%and 94.61%respectively and that in the combined wound group obviously decreased(t=7.84,7.26,8.18,P< 0.01) and was 52.67%,69.18%and 82.88%respectively.On the 1st,3rd and 5th day after burn,the quantity of inflammatory cells in the combined wound group decreased 26.53%,62.11%and 38.91%respectively compared with the control group,that of fibroblast decreased 43.73%,52.70%and 25.32%and that of blood capillary decreased 55.97%5 days after injury.After using Kangfuxin,step up of wound healing and increasing of cell quantity could be seen in both groups,but it was better in the combined group than the control group.On the 3rd and 5th day after injury,the bFGF content of epidermic cell in the combined group was lower 59.15%and 36.15%compared with the simple wound group.After using Kangfuxin,the width of increasing in the combined wound group increased 35.90%and 10.97%respectively than the single wound group.Conclusion Decreasing of local cell quantity in wound after injury and reducing of bFGF content in epidemic cells is one of the important factors causing the difficult recovery of wound surface.Kangfuxin has improving effect on the migration and proliferation of cells and the function of epidemic cell's excreting bFGF.

关 键 词：全身放射损伤 皮肤伤口 组织细胞 康复新 促愈作用 成纤维细胞 毛细血管数量 碱性成纤维细胞生长因子 

分 类 号：R818[医药卫生—放射医学]