苦参碱脂质体抗鸭乙型肝炎病毒的体内实验研究  

An Experimental Study of In - Vivo Effect of Matrine Liposome for Duck Hepatitis B Virus

作　　者：李常青[1] 张冬青[2] 李小翚[2] 张奉学[1] 周红燕[1] 程怡[2] 李向阳[1] 

机构地区：[1]广州中医药大学热带医学研究所,广东广州510405 [2]广州中医药大学中药学院,广东广州510405

出　　处：《新中医》2003年第6期77-78,共2页New Chinese Medicine

基　　金：广东省自然科学基金资助项目(编号:990353/B);广东省中医药管理局资助项目(编号:099517/A)

摘　　要：目的:观察苦参碱脂质体对鸭乙型肝炎病毒的影响。方法:对鸭乙型肝炎病毒(DHBV)实验感染的广州麻鸭雏鸭灌胃口服苦参碱脂质体和苦参碱,前者设20mg·kg^(-1)和10mg·kg^(-2)组,后者设20mg·kg^-组,每天1次,连续给药20天,另设病毒对照组。采用DHBV-DNA Dot Blot法,测定鸭血清DHBV-DNA水平值。结果:苦参碱脂质体20mg·kg^-组在给药后第10、15、20天鸭血清DHBV-DNA水平明显下降(P<0.01),效果优于苦参碱20mg·kg^(-1)组(P<0.05),与病毒对照组比较,差异有非常显著性意义(P<0.01)。苦参碱脂质体10mg·kg^(-1)组在给药后第20天及停药后3天鸭血清DHBV-DNA水平也有下降(P<0.05),与病毒对照组比较,差异有显著性意义(P<0.05),但与苦参碱20mg·kg^(-1)组比较,差异无显著性意义(P>0.05)。苦参碱脂质体2个剂量组停药后3天鸭血清DHBV-DNA水平无回升。结论:苦参碱脂质体对鸭乙型肝炎病毒有较好的抑制作用,优于同等剂量的普通苦参碱,其抗病毒效应相当于普通苦参碱1/2剂量的作用效果。Objective: To observe the in - vivo effect of matrine liposme (ML) on duck hepatitis B virus (DHBV). Methods: Young ducks model infected with DHBV was established. Four groups were observed: Group A by gastric perfusion of ML 20mg .kg-1; Group B by ML 10mg.kg-1; Group C by matrine 20mg. kg-1 and Group D (blank model). The drugs were given once daily for 20 days continuously. The levels of serum DHBV - DNA were determined by DHBV - DNA Dot Blot method. Results: After medication for 10, 15 and 20 days, the duck serum DHBV - DNA levels were markedly reduced in Group A (P < 0. 01), more marked than group C (P< 0. 05), and the difference was significant ( P < 0. 01) as compared with Group D. After 20 days medication and suspension for 3 days, the levels of duck serum DHBV - DNA in Group B were markedly reduced ( P < 0. 05), the difference being significant as compared with Group D( P < 0. 05), but not significant as compared with Group A( P > 0. 05). After suspension of the drug for 3 days, the levels of DHBV - DNA did not rise in both Group A and B. Conclusion: ML possesses an inhibitory effect on DHBV, which is more marked than the same dosage of matrine. Its anti - viral effect is equivalent to 1 /2 dosage of matrine.

关 键 词：苦参碱 脂质体 肝炎病毒乙型鸭 疾病模型 动物 鸭 

分 类 号：R285.5[医药卫生—中药学]