三线闭壳龟18SrRNA基因序列的测定  

DNA sequencing of 18S rRNA gene of Cuora trifasciata

作　　者：李贵生[1] 贾宗剑[1] 方堃[1] 唐大由[1] 

机构地区：[1]暨南大学生物工程学系,广州510632

出　　处：《生态科学》2003年第1期70-72,共3页Ecological Science

摘　　要：用分子遗传标记进行物种鉴别准确可靠,本文应用18S rRNA序列测定研究中药材三线闭壳龟的进化与种类鉴定。应用PCR直接测序技术测定三线闭壳龟肌肉18s rRNA基因部分核苷酸序列。结果表明,所测序列为678bp,其中GC占多数(54.1％)。讨论了DNA测序技术在龟鳖类等中药材鉴定方面的应用。It had been reported that the identification of species was accurate by genomic fingerprinting, so the evolution and identification of a Chinese traditional medicine Cuora trifasciata was studied by 18S ribosomal RNA gene sequences. The nuclear 18S rRNA gene region of muscles of Cuora trifasciata was amplified by PCR using a consensus primer set, and part of nucleotide was sequenced. The results showed that the length of 18S rRNA gene is 678 bp and GC is more abundance than AT. The application of DNA sequencing technique in the identify of Chinese traditional medicine was discussed.

关 键 词：三线闭壳龟 18SrRNA基因 序列测定 进化 分子遗传标记 物种鉴别 

分 类 号：Q959.63[生物学—动物学] Q953