实验性脾虚证大白鼠酶组织化学研究  

Studies on histochemistry of experimental syndrome of spleen insufficiency in rats

作　　者：张霞[1] 魏彦明[1] 杨其名 程富胜 程世红[4] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]兰州市动物检疫站 [3]兰州畜牧与兽药研究所 [4]兰州医学院

出　　处：《中兽医医药杂志》2003年第3期7-9,共3页Journal of Traditional Chinese Veterinary Medicine

基　　金：国家自然基金资助项目 ( 3 9770 5 5 8)

摘　　要：将 4 5只大白鼠随机分成 3组 ,即实验组、治疗组及对照组 ,每组 1 5只。以利血平致大白鼠脾虚证模型 ,分阶段捕杀动物 ,取肝脏、胃、十二指肠 ,用酶组织化学方法测定酸性磷酸酶 (ACP)、碱性磷酸酶 (AL P)、葡萄糖- 6 -磷酸酶 (G- 6 - Pase)活性的变化。结果表明 :实验组、治疗组、正常对照组各酶活性均发生了变化 ,在第 7天、第 1 4天时组内各酶存在着动态学上的变化。治疗组各酶活性接近正常对照组 。rats were divided randomly and averagely into three groups: experimental group, treatment group and control group. The model of syndrome of spleen insufficiency (ISS) was gained by injecting reserpine . The rats were killed at two stages. The liver, stomach and duodenum were sampled. The means of enzyme histochemistry was used in the experiment to test the changes of activities of acid phosphatase (ACP), glucose-6-phosphatase(G-6-Pase) and alkaline phosphatase(ALP). The result showed that the difference existed in above enzymes among groups and Sijunzi decoction had obvious treatment action.

关 键 词：实验性脾虚证 大白鼠 酶组织化学 酶活性 四君子汤 治疗作用 利血平 

分 类 号：R256.3[医药卫生—中医内科学] S853.33[医药卫生—中医学]