三七总皂甙对去大脑皮层血管后成年大鼠前脑侧脑室室管膜下层Nestin和bFGF表达的作用  

Effects of Total Saponin from Panax notoginseng on the Expressions of Nestin and bFGF in the Subependyma of the Forebrain Lateral Ventricle in Adult Rats with Cortical Devascularization

作　　者：程龙[1] 朱培纯[1] 司银楚[1] 吴海霞[1] 许红[1] 

机构地区：[1]北京中医药大学基础医学院解剖教研室,北京100029

出　　处：《北京中医药大学学报》2003年第3期18-20,共3页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金青年课题 (No .30 0 0 0 2 2 2 )

摘　　要：目的　探讨中药三七是否能够促进脑损伤后神经干细胞丝裂原bFGF的合成、分泌以及神经干细胞的增殖。方法　通过免疫组织化学的方法检测去大脑皮层血管后以及给予三七总皂甙后成年大鼠前脑室管膜下层 (SVZ)神经前体细胞标记Nestin和神经营养因子bFGF的表达。结果　给予三七总皂甙后去大脑皮层血管成年大鼠前脑侧脑室SVZ中Nestin标记细胞的数目明显增多 ,bFGF的表达明显增强。结论　三七总皂甙能促进脑内bFGF合成及分泌 。Objective To investigate whether the total saponin from Panax notoginseng (TSPN) can promote the synthesis and secretion of bFGF and the proliferation of the neural stem cells after cerebral injury. Method To detect the expressions of Nestin and bFGF in the subependyma of the forebrain lateral ventricle in the adult rats with cortical devascularization before and after the TSPN administration, by the immunohistochemical techniques. Results Both the number of Nestin and the expression of bFGF in the subependyma of the forebrain lateral ventricle were markedly increased after the rats with cortical devascularization were give TSPN. Conclusion TSPN can promote the synthesis and secretion of bFGF in the subependyma of the forebrain lateral ventricle, which may further stimulate the proliferation of the neural stem cells.

关 键 词：三七总皂甙 神经干细胞 细胞增殖 室管膜下层 去大脑皮层血管 

分 类 号：R285.5[医药卫生—中药学]