通脉宁胶囊对家兔颈动脉球囊损伤后血管再狭窄的影响  

Effects of Tongmaining Capsules on Vascular Restenosis After Carotid Balloon Injury in Rabbits

作　　者：石雪迎[1] 赵凤志[2] 宋崇顺[2] 戴欣[2] 陈淑长[3] 葛辛[3] 

机构地区：[1]北京大学医学部基础医学院病理学系,北京100083 [2]北京中医药大学东直门医院,北京100700 [3]北京中医药大学东方医院,北京100078

出　　处：《北京中医药大学学报》2003年第3期24-27,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家教委高等学校博士学科点专项科研基金 (No .2 0 0 0 2 6)

摘　　要：目的　观察通脉宁胶囊对经皮腔内血管成形术 (PTA)术后再狭窄的预防作用 ,并初步探讨其作用机制。方法　采用家兔颈动脉球囊损伤法复制PTA术后再狭窄模型 ,动物分为 5组 (n =10 ) :假手术组 ,对照组及通脉宁大、中、小剂量组 ,假手术组仅结扎颈外动脉 ,不插入气囊 ,其余 4组术后分别灌胃 0 5 %羧甲基纤维素钠混悬液 10mL/ (kg·d) ,通脉宁胶囊 1 64、0 82、0 4 1g/ (kg·d) ,连续 2 1d。第 2 1天给药 1h后处死 ,取出颈内动脉 ,肉眼、苏木素伊红染色观察动脉壁形态改变 ,masson染色以及免疫组化染色检测血管壁胶原形成及血管内皮生长因子 (VEGF)和碱性成纤维细胞生长因子 (bFGF)表达情况 ,图像分析系统进行半定量分析。结果　PTA后 2 1d ,对照组家兔颈动脉内膜明显增厚 ,平滑肌细胞 (SMCs)增生 ,中膜亦有一定程度增厚 ,血管壁胶原增多 ,同时管壁VEGF和bFGF表达增强 (与假手术组比较P <0 0 1)。通脉宁大剂量组内膜及中膜厚度明显降低 ,管壁胶原减少 ,VEGF和bFGF表达下降 (与对照组比较P <0 0 5 ,P <0 0 1) ,但仍高于假手术组 (P<0 0 1)。通脉宁中、小剂量组对上述部分指标有轻微的影响 ,但与对照组比较无明显差异 (P >0 0 5 )。结论　大剂量通脉宁对PTA后再狭窄有一定的预防作用 ,可能与其降低血?Objective To observe the preventive effects of Tongmaining Capsules (TC) on the restenosis after percutaneous transluminal angioplasty (PTA) and initially investigate the mechanism of the effects of TC. Methods The rabbit model of the restenosis after PTA was established by carotid balloon injury method. The rabbits were randomly divided into 5 groups (10 animals in each group), namely the sham operation, control, TC large dose, TC middle dose, and TC small dose. The animals in the sham group were only given external carotid artery ligation without balloon injury. After the carotid balloon injury, the animals in the rest 4 groups were intragastrically given 0.5% suspension of sodium carboxymethyl cellulose, 10 ml/kg·d -1; TC, 1.64 g/kg·d -1; TC, 0.82 g/kg·d -1; and TC, 0.41 g/kg·d -1, respectively; and all the doses were given successively for 21 days. 1 Hour after the administration on the 21st day, the animals in each group were executed and their internal carotid arteries were isolated for observing the morphological changes of the carotid artery wall by macroscopical observation and hematoxylin-eosin staining, and for detecting collagen formation of the vascular wall and the expressions of VEGF and bFGF by Masson staining and immunohistochemical techniques, with the help of an image analysis system to make semiquantitative analyses. Results On the 21st day after undergoing PTA, the carotid arteries of the rabbits in the control group showed markedly thickened tunica intima and smooth muscle cell proliferation, rather thickened tunica media and an increase in vascular wall collagen, and increased expressions of VEGF and bFGF as well, as compared with the sham operation group (P<0.01). The large dose of TC (1.64 g/kg·d -1) could markedly reduce the thickness of the tunica intima and tunica media, lessen the collagen in the carotid artery wall, and decrease the expressions of VEGF and bFGF, as compared with the control group (P<0.05, P<0.01), but the values of these criteria were still hig

关 键 词：通脉宁胶囊 血管成形术 再狭窄 家兔 

分 类 号：R285.5[医药卫生—中药学]