慢性移植物抗宿主病模型的诱导及补肾清热毒方的疗效观察  

Inducement of Chronic Graft Versus Host Disease (GVHD) Model and Observation of the Effects of Bushenqingredu Formula

作　　者：任文英[1] 陈香美[2] 邱全瑛[3] 陈扬荣[1] 王新高 师锁柱[2] 王兆霞[2] 

机构地区：[1]福建省人民医院,福建350003 [2]解放军总医院肾病实验室,北京100853 [3]北京中医药大学,北京100029 [4]华中科技大学同济医学院,湖北430003

出　　处：《北京中医药大学学报》2003年第3期31-34,69,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：福建省卫生厅科研课题 (No.70 2 )

摘　　要：目的　观察慢性移植物抗宿主病模型的诱导及补肾清热毒方的疗效。方法　选用 (DBA/ 2×C5 7BL/ 6J)F1小鼠 ,通过尾静脉注射其母鼠DBA/ 2淋巴细胞来诱导模型 ,观察 12周。同时于诱导后第 4周 ,将模型小鼠随机分为中药组 ,强的松组 ,模型组及未诱导的F1对照组 ,用药 8周后处死。观察自身抗体、血肌酐、尿素氮、肾脏病理等指标。结果　注射后 4周的小鼠仅有系膜细胞轻度增生 ,未见间质损害。 12周各项观察指标改变最明显 ,病理改变以内皮下大量嗜复红物质沉积 ,肾小管蛋白管型和肾间质大量炎细胞浸润 ,局灶或节段肾小球出现硬化为主要表现。免疫荧光示IgG、C3沿毛细血管沉积。中药组和强的松组均能降低尿蛋白浓度、自身抗体、血肌酐、尿素氮 ,中药组还有降低胆固醇功效 ,两药对轻度系膜细胞增生有效。结论　慢性移植物抗宿主模型类似人类狼疮性肾炎 ,是良好的狼疮肾炎模型。Objective To induce chronic graft versus host disease (GVHD) model and observe the effects of Bushenqingredu Formula (BF) on the disease model. Methods The chronic GVHD model was induced in F1 mice (DBA/2×C57BL/6j) by injecting their female parent's DBA/2 lymphocytes through the caudal veins, and the model was put under observation for 12 weeks. 4 Weeks after the model inducement, the mice were randomly divided into TCM drug group, prednisone group, model group. The control group was composed of the F1 mice not undergone the model inducement. 8 Weeks after the drug administration, the criteria such as auto-antibody, serum creatinine, blood urea nitrogen, and renal pathologic changes were observed in the mice after their execution. Results Only mild proliferation of mesangial cells was found and no interstitial damage was seen 4 weeks after the model inducement; while 12 weeks after the model inducement, changes in the pathologic criteria under observation were remarkable, mainly characterized by a large amount of subcutaneous fuchsinophilic deposites, renal tubular protein casts and a large area of infiltration by inflammatory cells in the renal interstitium, and focal or segmental glomerular sclerosis; and immunofluorescence showed that IgG and C3 deposited along capillary blood vessels. Both BF and prednisone could decrease the levels of urinary protein, auto-antibody, serum creatinine and blood urea nitrogen; BF also showed a cholesterol-reducing effect. Both BF and prednisone were effective for treating mild proliferation of mesangial cells. Conlusion The mouse model of chronic GVHD, which is alike to human lupus nephritis, can be used as a good model of lupus nephritis. BF exerts a curative effect in the early stage of lupus nephritis.

关 键 词：慢性移植物抗宿主病 狼疮肾炎模型 补肾清热毒方 疗效 

分 类 号：R285.5[医药卫生—中药学]