猪链球菌2型溶菌酶释放蛋白诱导上皮细胞融合和凋亡  

Fusion and Apoptosis of Epithelial Cells Induced by Muramidase Released Protein of Streptococcus suis Type 2

作　　者：曾巧英[1] 陆承平[1] 

机构地区：[1]南京农业大学农业部动物疫病诊断与免疫重点开放实验室

出　　处：《微生物学报》2003年第3期407-412,共6页Acta Microbiologica Sinica

基　　金：国家 973项目子课题 (G19990 1190 6)~~

摘　　要：猪链球菌 2型 (SS2 )溶菌酶释放蛋白 (MRP)的致病作用迄今不明 ,为此 ,以HEp 2细胞为上皮细胞体外模型 ,将纯化的MRP溶液和细胞共孵育 ,光镜观察 ,发现MRP可诱导细胞发生两种典型形态学变化 :一是诱导细胞融合形成多核巨细胞 ,随后巨细胞发生凋亡 ;二是诱导单个细胞凋亡。透射电镜观察和流式细胞分析确证MRP具有诱导上皮细胞凋亡的功能 ,凋亡率达 1 8%。证明MRP可单独作为SS2的毒力因子。The pathogenic role of muramidase released protein (MRP),a virulence factor of Streptococcus suis type 2 (SS2) is poorly understood. The purified MRP was co-incubated with HEp-2 cells to determine the effect of MRP on epithelial cells. Under light microscope, two principal morphologic changes were observed. Firstly, the cells were fused to form syncytia and a apoptosis followed. Secondly, single cell was also induced to apoptosis at high level as 18%, which was verified by transmission electron microscopy and flow cytometry. It showed that MRP alone was capable of a vi- rulence factor of SS2.

关 键 词：猪链球菌 溶菌酶释放蛋白 诱导 上皮细胞融合 细胞凋亡 

分 类 号：S852.61[农业科学—基础兽医学]