苏云金芽孢杆菌4.0718菌株的杀虫晶体蛋白基因分析  

Characterization of Insecticidal Crystal Proteins Genes from Bacillus thuringiensis 4.0718 Strain

作　　者：丁学知[1] 刘全兰[1] 莫湘涛[1] 高必达[2] 夏立秋[1] 

机构地区：[1]湖南师范大学生命科学学院微生物学系,长沙410081 [2]湖南农业大学植物保护学院,长沙410128

出　　处：《微生物学报》2003年第3期413-417,共5页Acta Microbiologica Sinica

基　　金：国家自然科学基金项目 (30 2 70 0 37);国家"863计划"项目 (2 0 0 2AA2 4 50 2 1);华中农业大学农业部农业微生物重点实验室项目 (3- 0 2 - 1- 2 1)~~

摘　　要：In this study, we rapidly identified Bacillus thuringiensis 4.0718 strain that harbored the known cry1 and cry2 type genes by a PCR strategy. Three pairs of universal oligonucleotide primers were designed to detect all known cry1, cry2 and cry3 type gene sequences. Then the DNA of the positive strain 4.0718 was probed with a set of specific primers. One feacture of this screening method was that each gene was expected to produce a PCR product having a precise molecular weight. PCR products having different sizes probably represented the gene was a potentially novel gene. Differentiations among these genes was determined on the basis of the electrophoresis patterns of PCR products. Finally, five cry1 type genes (cry1Aa, cry1Ab, cry1Ac, cry1Cb, a novel cry4.5 type genes) and one cry2Ac type gene had been detected from Bacillus thuringiensis 4.0718 strain.In this study, we rapidly identified Bacillus thuringiensis 4.0718 strain that harbored the known cry1 and cry2 type genes by a PCR strategy. Three pairs of universal oligonucleotide primers were designed to detect all known cry1, cry2 and cry3 type gene sequences. Then the DNA of the positive strain 4.0718 was probed with a set of specific primers. One feacture of this screening method was that each gene was expected to produce a PCR product having a precise molecular weight. PCR products having different sizes probably represented the gene was a potentially novel gene. Differentiations among these genes was determined on the basis of the electrophoresis patterns of PCR products. Finally, five cry1 type genes (cry1Aa, cry1Ab, cry1Ac, cry1Cb, a novel cry4.5 type genes) and one cry2Ac type gene had been detected from Bacillus thuringiensis 4.0718 strain.

关 键 词：苏云金芽孢杆菌 4.0718菌株 杀虫晶体蛋白基因 CRY基因 PCR 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]