虎杖的水溶性成分研究  

Studies on water-soluble constituents in rhizome of Polygonum cuspidatum

作　　者：肖凯[1] 宣利江[1] 徐亚明[1] 白东鲁[1] 

机构地区：[1]中国科学院上海生命科学研究院上海药物研究所,上海200433

出　　处：《中草药》2003年第6期496-498,共3页Chinese Traditional and Herbal Drugs

摘　　要：目的　研究虎杖的水溶性活性成分。方法　利用反相色谱的方法分离纯化 ,根据化合物的化学性质与光谱数据鉴定其结构 ,并测试其生物活性。结果　虎杖根茎的 6 0 %丙酮提取物中分得 6个化合物 ,确定其结构分别为白藜芦醇 ( ) ,云杉新苷 (白藜芦醇 - 3- O- β- D-吡喃葡萄糖苷 ) ( ) ,2 ,3-二氢 - 2 - (4′- O- β- D-吡喃葡萄糖基 - 3′-甲氧基 -苯基 ) - 3-羟甲基 - 5 - (3-羟基丙基 ) - 7-甲氧基苯唑呋喃 ( ) ,2 ,6 -二甲氧基 - p-苯 - 1- O- β- D-吡喃葡萄糖苷 ( ) ,5 ,7-二羟基 -异苯唑呋喃 ( ) ,5 ,7-二羟基 -异苯唑呋喃 - 7- O-β- D-吡喃葡萄糖苷 ( )。结论　化合物 ～ 为首次从该植物中分得 ,化合物 ～ 没有显示出 DNA裂解活性 ,化合物 对 KB和 MCF- 7细胞表现出较弱的细胞毒活性。Object To study the chemical structures and bioactivity of the water-soluble constituents from Polygonum cuspidatum Sieb. et Zucc. Methods To isolate the constituents by reverse phase methods, and characterize their structures by the analysis of chemical property and spectral data. Results Six compounds were isolated from the 60% aqueous acetone extract from the rhizome of P. cuspidatum. Their structures were elucidated as reveratrol (Ⅰ); piceid (Ⅱ); 2, 3-dihydro-2-(4′-O-β-D-glucopyranosyl-3′-methoxy-phenyl)-3-hydroxymethyl-5-(3-hydroxypropyl)-7-methoxybenzofuran (Ⅲ); 2, 6-dimethoxy-p-hydroquinone-1-O-β-D-glucopyranoside (Ⅳ); 5, 7-dihydroxy-isobenzofuran (Ⅴ) and 5, 7-dihydroxy-isobenzofuran-7-O-β-D-glucopyranoside(Ⅵ), respectively. Conclusion Compounds Ⅲ-Ⅵ are isolated from the plant for the first time. Compounds Ⅰ-Ⅵ show no DNA cleavage activity. Compound Ⅱ exhibits weak cytotoxicity against two human cancer cell lines (KB and MCF-7) in vitro.

关 键 词：虎杖 水溶性成分 反相色谱法 生物活性 分离 纯化 

分 类 号：R284.1[医药卫生—中药学]