浙江产香茶菜属植物8个居群的RAPD分析  

RAPD analysis of eight populations in plants of Isodon (Schrad. ex Benth.) Kudo from Zhejiang Province

作　　者：方芳[1] 郭水良[2] 黄华[2] 毛晓燕[2] 郭卫东[2] 

机构地区：[1]浙江大学生命科学学院,浙江杭州310028 [2]浙江师范大学生命与环境科学学院,浙江金华321004

出　　处：《中草药》2003年第6期553-556,共4页Chinese Traditional and Herbal Drugs

基　　金：浙江省自然科学基金 (3 0 10 2 8)资助项目 ;金华市科委计划项目 (2 0 0 1-1-2 71)资助 ;浙江省中青年学科带头人基金资助项目

摘　　要：目的　从 DNA分子水平上探讨香茶菜属植物种群变异特点。方法　从 70个随机引物中筛选出 10个具有较高多态性检测能力的引物。用这 10个随机引物对浙江境内 3种香茶菜的 8个居群进行了 RAPD分析 ,对得出的RAPD结果应用 SPSS10 .0进行的聚类分析和应用 CANOCO3.10进行的除趋势对应分析。结果　共检测出 14 4个位点 ,其中单态位点 11个 ,占 7.6 4 % ,多态位点 133个 ,占 92 .36 %。分析结果表明 ,浙江产香茶菜不同种、同一种的不同居群间存在明显的遗传分化 ,其中种间的差异要大于种内 ,大萼香茶菜 Isodon macrocalyx居群间的差异要比香茶菜 I.amethystoides居群的小。结论　香茶菜形态与化学成分的变异有一定的遗传学基础。Object To study the population variations from plants of Isodon (Schrad. ex Benth.) Kudo at DNA level. Methods Eight plant populations of Isodon (Schrad. ex Benth.) Kudo collected from Zhejing Province were analyzed by RAPD. The results for eight populations from three species of Isodon (Schrad. ex Benth.) Kudo were analyzed by clustering analysis SPSS10.0 and Detrended Correspondence Analysis (DCA) CANOCO 3.10. Ten of the 70 random primers were tested to possess the stronger detecting effect of polymorphous character. Results A total of 144 bands was amplified by the 10 primers, 7.64% of the total bands was 11 monomorphic, 92.36% of the total bands was 133 polymorphic. The results indicated that the genetic variations existed within the populations of the same species or the different species in Zhejiang Province, and the variances within the populations of I. macrocalyx were slightly smaller than those of I. amethystoides. Conclusion There are some genetic backgrounds in the variations of the morphology and the chemical compounds in the species of I. amethystoides.

关 键 词：香茶菜属植物 居群 RAPD 遗传多样性 化学成分 

分 类 号：R284.1[医药卫生—中药学] R282.71[医药卫生—中医学]