光质和生长物质组合对怀山药零余子脱分化和再分化的影响  

The Effect of Combination of Light Quality and Plant Growth Substance on Dedifferentiation and Redifferentiation in the Bulbil of Dioscorea Opposita

作　　者：郭君丽[1] 陈明霞[1] 李明军[1] 张嘉宝[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453002

出　　处：《河南师范大学学报（自然科学版）》2003年第2期99-102,共4页Journal of Henan Normal University(Natural Science Edition)

基　　金：河南省自然科学基金项目 (编号 :9840 12 0 2 );河南省科技攻关资助项目 (编号 :0 12 3 0 3 0 90 0 )

摘　　要：本文以怀山药优良品种“铁棍山药”为材料 ,研究了光质和生长物质的不同组合对零余子脱分化和再分化的影响 .结果表明 :( 1)在愈伤组织诱导过程中 6-BA与NAA的组合优于 6-BA与 2 ,4-D的组合 ;在 6-BA2mg/L(单位下同 ) +NAA 2和 4培养基上 ,愈伤组织在白光和红光下出现的比较早 ,诱导率在白光、红光、黄光、蓝光、绿光及黑暗各条件下均能达到 10 0 % .( 2 )在愈伤组织分化过程中 ,在相同条件下的蓝光分化率最高 (为 10 0 % ) ,红光和白光次之 (分别为 95 %和 91.7% ) ,绿光和黄光较低 (分别为 84.4%和 75 % ) ,黑暗最低 (为 2 2 .6% ) .This paper deals with the effect of combination of light quality and plant growth substance on the dedifferentiation and redifferentiation in the bulbil of Tiegun Dioscorea Thund. Results showed that: During the callus induction, the combination of 6-BA and NAA is superior to that of 6-BA and 2,4-D; calli's production is earlier, the rate of callus production can be reached to 100% under all light quality. During differentiation of calli, under the same condition, the rate of differentiation is hightest in blue light (100%), in red light and white light (95%and 91.7%), in green light and yellow light (84.4% and 75%), and lowest in dark (22.6%).

关 键 词：怀山药 中药材 组织培养 愈伤组织 零余子 脱分化 再分化 光质 生长物质 

分 类 号：Q943.1[生物学—植物学] R282.71[医药卫生—中药学]