湖羊、同羊12个同工酶座位的检测  

Genetic estimation of 12 isozymes in Hu sheep and Tong sheep

作　　者：孙伟[1] 常洪[1] 任战军[2] 杨章平[1] 耿荣庆[1] TSUNODA Kenji 

机构地区：[1]扬州大学畜牧兽医学院,江苏扬州225009 [2]西北农林科技大学畜牧兽医学院,陕西杨陵712101 [3]Showa University School of Medicine

出　　处：《动物学报》2003年第1期134-138,共5页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金国际研究项目 (No .30 2 130 0 9)资助~~

摘　　要：Gene frequencies of Hu sheep and Tong sheep were obtained with“Random sampling in typical colonies of a central area”. Of the 12 loci tested in Hu sheep, 11 loci were polymorphic. Reliability of the estimated frequencies of 27 alleles reached 0.95 except for PoF, TfA,Tff,Hb-βA and CAF which had reliabilities of 0.5222,0.7478,0.5222,0.6212 and 0.899, respectively. Of the 12 loci tested in Tong sheep, 11 loci were polymorphic. Reliability of the estimated frequency of 25 alleles reached 0.95 except for TfA, TfE and CAF which had reliabilities of 0.931,0.6922 and 0.7924, respectively. The average heterozygosity () and average homozygosity (J) was computed and the J of the two sheep colonies was 0.6619 and 0.6448, respectively. Consistent with our conclusions based on genetic data, previous research divided the native sheep populations of East and South Central Asia into three group: the“Mongolian group”,“South-Asian group” and“European group”. Consequently, the degree of genetic similarity between populations and known groups would seem to provide a reliable means of determining the genetic relationships between populations and may reflect the true genetic origin of Hu sheep and Tong sheep in China [Acta Zoologica Sinica 49(1):134-138,2003].Gene frequencies of Hu sheep and Tong sheep were obtained with“Random sampling in typical colonies of a central area”. Of the 12 loci tested in Hu sheep, 11 loci were polymorphic. Reliability of the estimated frequencies of 27 alleles reached 0.95 except for PoF, TfA,Tff,Hb-βA and CAF which had reliabilities of 0.5222,0.7478,0.5222,0.6212 and 0.899, respectively. Of the 12 loci tested in Tong sheep, 11 loci were polymorphic. Reliability of the estimated frequency of 25 alleles reached 0.95 except for TfA, TfE and CAF which had reliabilities of 0.931,0.6922 and 0.7924, respectively. The average heterozygosity () and average homozygosity (J) was computed and the J of the two sheep colonies was 0.6619 and 0.6448, respectively. Consistent with our conclusions based on genetic data, previous research divided the native sheep populations of East and South Central Asia into three group: the“Mongolian group”,“South-Asian group” and“European group”. Consequently, the degree of genetic similarity between populations and known groups would seem to provide a reliable means of determining the genetic relationships between populations and may reflect the true genetic origin of Hu sheep and Tong sheep in China [Acta Zoologica Sinica 49(1):134-138,2003].

关 键 词：湖羊 同羊 同工酶 座位 检测 遗传检测 血统归属 

分 类 号：Q953[生物学—动物学]