复方丹参方预处理对心肌缺血再灌注损伤的保护作用  

Protective effect of fufang danshenfang pretreatment on myocardial ischemical reperfusion injury

作　　者：高秀梅[1] 张伯礼[1] 商洪才[1] 王怡[1] 郭利平[1] 张萌[1] 史红[1] 刘密新[2] 

机构地区：[1]天津中医学院中医药研究中心,天津市300193 [2]清华大学化学系,北京市100084

出　　处：《中国临床康复》2003年第12期1754-1756,共3页Chinese Journal of Clinical Rehabilitation

基　　金：国家重点基础研究发展规划项目---方剂关键科学问题研究(G1999054403)~~

摘　　要：目的观察复方丹参方预处理冠脉结所经典大鼠,验证其是否具有模拟或加强缺血预适应减少缺血再灌后折损伤后的作用。方法180只大鼠采用Stata统计软件随机分为9组,每组各20只。采用经典大鼠冠脉结扎,缺血5min,再灌5min,反复循环3次缺血性预适应模型,以及缺血30min,再灌2h的单纯缺血再灌模型,以坏死区占缺血区的百分比、恶性心律失常的发生率、心肌酶、自由基为评价指标。结果再灌组,早、晚期假性预适应组坏死质量占缺血质量(IS/AAR)分别高达45%,47%,45%;心率失常的发生率也分别高达76%,56%,73%;血清中LDH含量也显著高于对照组,P<0.01。缺血预处理后,早期组IS/AAR与早期假性预适应组相比降低了17%,晚期组IS/AAR与晚期假性预适应组相比仅降低4%;早期缺血预处理组和晚期缺血预处理组心率失常发生率分别为13%,44%,显著低于再灌和早晚期假性预适应组,P<0.01;缺血预处理之后LDH释放也减少。复方丹参方预处理后,在再灌、IPC基础上IS/AAR进一步缩小约10%～17%,LDH释放也减少,LDH含量以晚期药物+IPC组最低,与晚期假性预适应组相比P<0.05,复方丹参方使再灌组心率失常发生率下降。另外复方丹参方预处理后,还可以使血清中MDA下降,SOD活性增强。结论复方丹参方可加强缺血性预适应的效应,但对早期缺血性预适应的效应不及?Aim To study the effect of the Chinese herbal compound prescription ?(r)Fufangdanshenfang?ˉ(?(r)FFDSF’) in protection of myocardial ischemical reperfusion injuries in simulating and enhancing ischemic preconditioning (IPC). Methods 180 rats were divided into 9 groups of 20 rats each. Based on the classical rat model of ischemic preconditioning by the coronary artery ligation, ischemia preconditioning mode was cultured by ischemia for five minutes, reperfusion for five minutes, then repeating three times, while the simple ischemic model was ischemia for 30 minutes and reperfusion for 2 hours. The necrosis percentages of ischemia areas, frequencies of arrhythmia, changes of myocardium enzyme and free radical were assayed. Results In the reperfusion group, the ratios of the infarct size of the area at risk (IS/ARR) were 45%, 47%and 45%in the early, metaphase and late sham ischemic preconditioning (SIPC) groups respectively. The frequencies of arrhythmia were 76%, 56%and 73%respectively. The contents of serum LDH in all groups above were significantly higher than that of the control (P< 0.01). After the ischemic preconditioning, the necrosis percents of the early IPC group reduced 17%compared to that of the SIPC group. The ratio of IS/ARR in the late IPC group reduced 4%compared to that in the late SIPC group. The ventricular arrhythmia of the early and late IPC groups were 13%and 44%respectively, significantly lower than those of the reperfusion group and early and late SIPC groups (P< 0.01). The release of the LDH also reduced after the ischemic preconditioning. After pretreatment with FFDSF, the IS/ARR ratio further reduced about 10%?17%, as well as the release of the LDH. The content of the LDH was the lowest in the late group of drug plus IPC, with a significant difference from the late SIPC (P< 0.05). FFDSF reduced the incidence of arrhythmia and content of MDA, and increased the activity of SOD.Conclusion FFDSF enhances the effects of the IPC. Its effect is not better than that of the ischemic preconditioni

关 键 词：复方丹参方 心肌缺血 再灌注损伤 保护作用 大鼠 心肌酶 缺血预适应 

分 类 号：R285.5[医药卫生—中药学] R259.422[医药卫生—中医学]