RAPD技术在家蚕杂交率检验中的应用  

Purity determination on silkworm hybrid rate using RAPD technique

作　　者：钟伯雄[1] 张金卫 丁农 陶京亚[1] 陆云翀[1] 

机构地区：[1]浙江大学特种经济动物科学系,浙江杭州310029 [2]浙江湖州蚕桑科学研究所,浙江湖州313000

出　　处：《浙江大学学报（农业与生命科学版）》2003年第3期321-324,共4页Journal of Zhejiang University:Agriculture and Life Sciences

摘　　要：研究发现家蚕每一个体基因组的DNA片断的特异性分别具有物种、品种和个体3个层次,其中只有品种特异性的DNA片断才能作为家蚕杂交率检验的判别标志.用一粒蚕卵或一条蚁蚕抽提得到的DNA,与从丝腺细胞中抽提得到的DNA的PCR反应结果相同,故在卵期可利用RAPD技术简便、快速,正确地检验家蚕的杂交率.It was found that specifity of DNA fingerprinting of the genome from individual silkworm can be characterized on three different levels, namly, speciesspecific, varietyspecific and individualspecific level. Only pecularity of DNA that are varietiespecific can serve as juge criterion for purity determination on the hybrid. The same PCR result can be amplified by DNA template from a single silkworm egg or a newly hatched larva and from silkworm gland cells. So fingerprint based on DNA at the egg stage is promising to be used in rapidly assessing purity of hybrid.

关 键 词：RAPD技术 家蚕 杂交率 检验 应用 卵期 

分 类 号：S882.3[农业科学—特种经济动物饲养]