酶免疫法检测粪便中幽门螺杆菌抗原在临床感染中的价值  

Stool Specimem for Helicobacter pylori Antigen Tested by EIA: A Clinical Evaluation

作　　者：朱晓玲[1] 孙艳艳[1] 吴雪梅[1] 

机构地区：[1]北京市石景山医院,北京100043

出　　处：《中华医院感染学杂志》2003年第6期517-519,共3页Chinese Journal of Nosocomiology

摘　　要：目的　探讨酶免疫法 (EIA)检测幽门螺杆菌 (Helicobacterpylori,Hp)粪便抗原在Hp感染中的临床价值。方法　采用EIA法检测 94例患者粪便标本幽门螺杆菌抗原 ,部分同时进行14 C UBT、组织学方法的检测 ,以14 C UBT作为标准 ,对EIA法进行评估 ,同时与RUT试验、Warthin Starry银染法进行比较 ,对其方法的可靠性进行评估。结果　EIA法的敏感性为 94 .3%、特异性为 90 .2 %、准确性为 92 .6 %、阳性预测值为 92 .6 %、阴性预测值为 92 .5 % ,均高于其他测定方法。结论　EIA法检测Hp抗原的敏感性、特异性、准确性均较好 ,适合临床实验室作为常规项目开展 ,可用于幽门螺杆菌感染的筛选和诊断 ,也适用于Hp的根除疗效观察和随访复查。OBJECTIVE To investigate the clinical value on stool specimen for Hp antigen tested by EIA. METHODS The stool specimen for Hp antigen specimens from 94 patients were tested by EIA, 14C-UBT and histologic method. The test results of 14C-UBT were regarded as the standard, and the results of EIA, RUT and Warthin-Starry silver stain method were compared and evaluated. RESULTS The sensitivity of EIA was 94.3%, specificity 90.2%, accuracy 92.6%, The positive predictive value was 92.6% and negative predictive value 92.5% which were all higher than those of other methods. CONCLUSIONS The EIA method for Hp antigen testing is better in high sensitivity specificity and accuracy than other methods, and fit for Hp screening and diagnosis in regular clinical detection.

关 键 词：酶免疫技术 粪便 幽门螺杆菌 抗原 

分 类 号：R378.2[医药卫生—病原生物学]