新分离的冠状病毒与严重急性呼吸综合征病原关系的研究  

STUDY ON THE ASSOCIATION BETWEEN A NOVEL CORONAVIRUS AND THE SARS PATHOGEN

作　　者：祝庆余[1] 秦鄂德[1] 于曼[1] 司炳银[1] 杨保安[1] 刘洪[1] 吕富双[1] 常国辉[1] 彭文明[1] 范宝昌[1] 邓永强[1] 韩伟国[1] 石玉玲[2] 李林海[2] 张泮河[1] 赵秋敏[1] 曹务春[1] 

机构地区：[1]军事医学科学院微生物流行病研究所 [2]广州军区广州总医院

出　　处：《解放军医学杂志》2003年第6期477-479,共3页Medical Journal of Chinese People's Liberation Army

摘　　要：目的　确定新分离的冠状病毒与目前流行的严重急性呼吸综合征的病原关系。方法　以用细胞培养法从严重急性呼吸综合征病例标本中分离出的冠状病毒为抗原 ,通过免疫荧光染色及中和试验测定严重急性呼吸综合征患者血清中抗新分离的冠状病毒的抗体 ,分析确定新冠状病毒与严重急性呼吸综合征的病原关系。结果　在临床确诊的 1 1 3份严重急性呼吸综合征患者血清标本中 ,99份检测出有抗新冠状病毒的抗体。 1 0对双份血清检测结果显示 ,后采集的血清抗体效价均比发病初期采集的明显增高 ,最高的升高 1 2 8倍。中和试验结果证明 ,病人血清抗体能够中和新分离的冠状病毒。结论　新分离的冠状病毒与此次流行的严重急性呼吸综合征密切相关 。Objective To determine the causative association between a newly isolated coronavirus and the current epidemic of severe acute respiratory syndrome (SARS) Methods Coronavirus was isolated from the samples of patients with SARS by cell culture Immunofluorescence assay and neutralization test were used to detect the antibodies in serum of SARS patients against newly isolated coronavirus, in order to analyze and determine the association between coronavirus and SARS pathogen Results The antibodies against the novel coronavirus could be detected in 99 of 113 sera from clinically diagnosed SARS patients The results of 10 pairs of double serum detection showed that, antibody titers in the late phase were significantly higher than those in the acute phase, and a highest increase by 128 folds was being found. The neutralization test showed that the antibodies from SARS patients' sera could neutralize the novel coronavirus Conclusion The newly isolated coronavirus was closely associated with and possibly the key pathogen of SARS

关 键 词：严重急性呼吸综合征 SARS 新冠状病毒 病原关系 中和试验 

分 类 号：R563.1[医药卫生—呼吸系统]