广州地区呼吸道合胞病毒分离株G蛋白基因序列分析  

Sequence analysis of G protein gene of human respiratory syncytial virus in Guangzhou area

作　　者：阳隽[1] 黄海鹭[2] 徐军[2] 钟南山[2] 

机构地区：[1]广州医学院第二附属医院呼吸内科,广州510260 [2]广东省广州呼吸疾病研究所,510120

出　　处：《广东医学》2003年第6期580-582,共3页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目 (编号 :39870 32 5)

摘　　要：目的　探讨广州地区呼吸道合胞病毒 (RSV)G蛋白基因变异的生物学意义 ,为研究广州地区RSV感染特点及RSV疫苗提供依据。方法　用PT -PCR的方法从广州地区呼吸道合胞病毒分离株 9815 9株的细胞培养物中扩增出编码其表面蛋白G的基因片段 ,克隆至pGEM -T载体中并进行序列测定。结果　序列测定结果与A亚型原型株A2株、1990年中国北京地区分离株B79G蛋白的核苷酸序列比较核苷酸同源率分别为 92 7%及 98 4% ;由核苷酸序列推导的氨基酸序列的同源率分别为 88 3 %及 97 0 %。一些重要的免疫反应位点发生了变异。结论　RSVG蛋白抗原结构变异大 。Objective To study the sequence of G protein gene of human respiratory syncytial virus in Guangzhou area of China. Methods The cDNA encoding RSV G protein gene was amplified from HEP-2 cells infected RSV by RT-PCR and cloned into pGEM-T vector. The recombinant plasmid was sequenced. Results The nucleotide sequence for RSV G protein presented 92 7% and 98 4% overall nucleotide identity compared with those of RSV isolate A2 and B79 strain respectively. The deduced amino acid sequence of the cloned G protein presented with 88 3% and 97 0% overall amino acid identity compared with those of isolate A2 and B79 strain respectively. Variations of amino acid were found in some antigen epitopes. Conclusion Field RSV isolates may be optimal candidate for preparing vaccines in different areas.

关 键 词：广州地区 呼吸道合胞病毒 分离株 G蛋白 基因序列分析 

分 类 号：R373.1[医药卫生—病原生物学]