流动注射荧光法测定羟基自由基  

Determination of Hydroxyl Radical by Flow Injection Fluorometry

作　　者：刘立明[1] 宋功武[1] 刘丽虹[1] 方光荣[1] 

机构地区：[1]湖北大学分析测试中心,武汉430062

出　　处：《分析化学》2003年第6期723-725,共3页Chinese Journal of Analytical Chemistry

摘　　要：研究Sn2 + 催化H2 O2 产生的·OH的反应 ,·OH与Ce3+ 作用后氧化生成无荧光的Ce4 + 。通过测定Ce3+ 的荧光强度的变化可间接测定所产生的羟自由基 ,并结合流动注射技术 ,确定了体系最佳实验条件。测定抗氧化剂清除羟自由基的实验 ,证明该体系可作为在线筛选抗氧化剂的方法之一。A new method was developed for determination of the hydroxyl radical in the Sn2+ -H2O2 system by flow injection fluorometry: Ce3+ could produce its characteristic fluorescence in diluted sulfuric acid and its fluorescence was quenched when Ce3+ was oxidized by hydroxyl radical. With measuring of fluorescence intensity, the content of hydroxyl radical in the solution was determined. The optional experimental program was obtained by studying the determination conditions. The results of clearing experiment showed the method can be applied to sieve the antioxidants on line.

关 键 词：流动注射 荧光法 测定 羟基自由基 抗氧化剂 筛选 

分 类 号：O657.3[理学—分析化学]