高灵敏度电容型透明质酸结合蛋白免疫传感器的研制  

A High Sensitive Hyaluronan Binding Protein Capacitive Immunosensor

作　　者：江德臣[1] 黄松 唐佳[1] 刘宝红[1] 黄宜平[3] 孔继烈[1] 

机构地区：[1]复旦大学化学系,上海200433 [2]上海申能德赛诊断技术有限公司,上海200433 [3]复旦大学微电子学系,上海200433

出　　处：《分析化学》2003年第6期713-715,共3页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金 (No .2 0 0 0 5 0 0 2 ;3 0 2 70 3 81);"973"项目 (No .0 0 1CB5 10 2 0 2 );上海市青年科技启明星计划;复旦大学交叉项目(No .0 1QA14 0 0 7)

摘　　要：实验用金电极自组装技术构建透明质酸结合蛋白电容型免疫传感器 ,采用交流阻抗技术对电极表面生物绝缘膜进行研究。按R(RC)等效电路图对其阻抗谱进行拟合的偏差较小 ,显示实际体系接近于拟合的电路图。该传感器对 1～ 10 0mg L抗原具有良好的线性响应。构建的生物膜对于一定范围的离子强度、酸度具有良好的稳定性 。Hyaluronan binding protein (HABP) capacitive immumosensor was fabricated with self-assembled technology and studied with impedance spectroscopy in the absence of Faradaic reaction. The deviation of fitting based on R (RC) circuit was little, which indicated that the system was similar to the model. In the range of 1 100 mg/L, the immunosensor showed a good linear response to HABP antigen, Increasing the ion strength and the acidity of the electrolyte, the monolayer was stable without any pinholes. This made the immunosensor be promising in the clinical application.

关 键 词：透明质酸结合蛋白 自组装技术 交流阻抗 电容型免疫传感器 病理分析 临床检测 巯基乙酸 金电极 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]