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机构地区:[1]江苏省中科院植物研究所江苏省植物迁地保护重点实验室,江苏南京210014
出 处:《林产化学与工业》2003年第2期68-72,共5页Chemistry and Industry of Forest Products
基 金:国家自然科学基金资助项目(30270108);江苏省农业科技攻关项目(BE2001356)
摘 要: 应用反相高效液相色谱法(RP HPLC)对中国重要的甾体激素药源植物盾叶薯蓣具有代表性的9个野生居群和3个栽培居群的薯蓣皂苷元含量进行了测定,分析了经引种至江苏南京栽培1a后各居群薯蓣皂苷元含量变化、根茎的年生长量以及根茎含水量。结果表明:薯蓣皂苷元含量及其栽培前后含量变化与盾叶薯蓣种质有关,不同种质之间差异较大;栽培居群根茎生长量明显高于野生居群。薯蓣皂苷元含量及其变化与根茎中水分含量无明显相关性。薯蓣皂苷元含量的遗传稳定性应作为优良种质筛选的首要标准。By RPHPLC method,diosgenin content of Dioscorea zingiberensis collected from 9 wild populations and 3 cultured populations in China were determined.The change of diosgenin content and weight of rhizoma were also tested after introduced from their native sites to Nanjing,Jiangsu Province and cultivated for a year.The result showed that diosgenin content in a year and the annual enlargement of root vary among different populations.D.zingiberensis of cultured populations has significant root enlargement and less diosgenin content than those of wild populations.In addition,the diosgenin content and its change are not correlated with water content.It concludes that the hereditary maintance of diosgenin content should be considered as the principal criterion in selection of cultivar.
关 键 词:中国 盾叶薯蓣 反相高效液相色谱法 薯蓣皂苷元 居群 种质分析 药用植物 良种筛选
分 类 号:Q949.95[生物学—植物学] S567[农业科学—中草药栽培]
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