蜡蚧轮枝菌对烟粉虱的时间-剂量-死亡率模型研究  

The time-dose-mortality model of two Verticillium lecanii isolates on sweetpotato whitefly, Bemisia tabaci

作　　者：王联德[1] 黄建[1] 林谷园[1] 梁智生[1] 

机构地区：[1]福建农林大学植物保护学院,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2003年第2期167-171,共5页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金资助项目(39970511;30270904).

摘　　要：以叶片浸液接种法测定了烟粉虱对蜡蚧轮枝菌菌株(Vp28和Vl6063)的感染反应.运用时间、剂量、死亡率模型对死亡率随时间和剂量的变化作了拟合.接种后4-8d,Vl6063的LC50分别为3.98×108,3.55×107、6.31×106、1.17×106、6.03×105孢子·mL-1;Vp28的LC50分别为6.61×106,1.95×106、1.10×106、7.76×105和6.03×105孢子·L-1.2个菌株的LT50值为105-106孢子·mL-1(约为5.5-10.0d).在小于105孢子·mL-1的剂量下,Vp28的LT50值大于Vl6063;在大于106孢子·mL-1剂量下,Vl6063的LT50值则大于Vp28、Vp28的.Bioassays of 2 isolates (Vp28, Vl6063) of Verticillium lecanii (Zimm.) were conducted on Bemisia tabaci. For inoculation, batches of 3rd instar whitefly nymphs on sweet potato seedlings were immersed in conidial suspensions at the content of 103-107 conidia·mL-1. The nymphs were maintained at 25 ℃, under 90% RH, photo phase of 16∶8 (L∶D) and observed daily for mortality. The resulting timedosemortality data fitted well to a conditional mortality probability model based on the HosmerLemeshow test, resulting in a cumulative mortality probability model.　The parameters from the latter model were used to estimate the lethal concentration (LC50) and time (LT50) for two isolates against Bemisia tabaci. The LC50 values for Vl6063 were 3.98×108,3.55×107,6.31×106,1.17×106,6.03×105 conidia·mL-1 on 4-8 d after inoculation, and the values for Vp28 were 6.61×106,1.95×106,1.10×106,7.76×105,6.03×105 conidia·mL-1 on 4-8 d after inoculation, respectively. The estimates of LT50 values were similar at the dosage of 105-106 conidia·mL-1 for two isolates. The results indicated that isolate Vl6063 from Canada was also a promising microbial agent as isolate Vp28 for whiteflies in Fuzhou, China.

关 键 词：烟粉虱 蜡蚧轮枝菌 时间—剂量—死亡率模型 叶片浸液接种法 生物测定 昆虫病原真菌 生物防治 

分 类 号：S433.3[农业科学—农业昆虫与害虫防治] S476.12[农业科学—植物保护]