动物组织中磺胺二甲嘧啶残留检测ELISA试剂盒的研制  

Development of an enzyme linked immunosorbent assay kit for sulfamethazine in animal tissues

作　　者：沈建忠[1] 何方洋[1] 何继红[1] 王兆芹 张航 江海洋[1] 冯才伟 杨云贵 

机构地区：[1]中国农业大学动物医学院,北京海淀100094 [2]北京望尔生物技术有限公司,北京海淀100094

出　　处：《中国兽医杂志》2003年第6期6-8,共3页Chinese Journal of Veterinary Medicine

基　　金："十五"国家科技攻关课题(2002BA514A-14)

摘　　要：在建立竞争ELISA方法的基础上,首次研制出检测磺胺二甲嘧啶的单克隆抗体快速检测试剂盒,并对其检测限、精密度、检测范围以及鸡肌肉组织中的添加回收实验做了详细研究。本试剂盒的检测限为1.0ng/ml,检测范围为1.0～81.0ng/ml,批内变异系数<8.9％,批间变异系数<9.5％,在10、60和200ng/ml水平鸡肌肉组织中添加,回收率为64.5％～85.5%,变异系数为6.0％～18.6%。与同类相关德国产试剂盒相比较,阳性符合率为100％。A sensitive and specific ELISA for SM2was established by anti-sulfamethazine monoclonal antibody. In the assay,sulfamethazine residue in sample was allowed to compete with the sulfamethazine-OVA conjugate immobilized on microtiter wells for binding to a limited amount of the anti-sulfamethazine monoclonal antibody, Horseradish peroxidase coupled to the goat IgG of anti-mouse IgG was selected as a tracer. Standard curves were constructed over 1 - 81 ng/ml of sulfamethazine,and the limit of detection of the ELISA was 1 ng/ml. Recoveries of sulfamethazine in spiked samples between 10, 60 and 200 ng/ml ranged from 64. 5% to 85. 5%. Precision of the assay as determined by interassay and intraassay variabilities was below 10%. Among 6 sulfonamide analogues tested for specificity .Sulfadimethoxine and sulfamer-azine showed significant cross-reaction in the assay. The assay is rapid,sensitive,and convenient to perform, and the test is readily adaptable to high-volume testing of sample in slaughterhouses.

关 键 词：动物组织 磺胺二甲嘧啶 药物残留 检测技术 ELISA试剂盒 研制技术 

分 类 号：S859.84[农业科学—临床兽医学]