植物雌激素大豆苷原(daidzein)对结肠癌LoVo细胞生长的影响  

Effect of daidzein on the growth of LoVo cells in vitro

作　　者：赖依峰[1] 郭俊明[2] 肖丙秀[2] 陈枢青[1] 

机构地区：[1]浙江大学医学院,浙江杭州310009 [2]宁波大学医学院,浙江宁波315211

出　　处：《中国现代应用药学》2003年第3期176-178,共3页Chinese Journal of Modern Applied Pharmacy

基　　金：宁波大学人才启动基金 (0 14 -4 710 3 6);浙江省教育厅科研基金 (2 0 0 10 2 17)

摘　　要：目的　了解植物雌激素大豆苷原 (daidzein)对结肠癌细胞生长的影响。方法　取对数生长期的人结肠癌细胞株LoVo细胞 ,以不同浓度的大豆苷原处理细胞 2 ,3,4和 6d ,然后应用MTT法测定细胞的生长率。结果　大豆苷原在高浓度 10 0 μmol/L时在体外对LoVo有明显的抑制作用 (P <0 .0 1) ,而在低浓度 <5 μmol/L时对LoVo细胞又具有促增殖作用。OBJECTIVE To study the effect of daidzein, one of the phytoestrogen,on the growth of human colonic cancer cell line LoVo cells. METHOD LoVo cells were cultured in RPMI 1640 cantaining 10% fetal bovine serum and were incubated at 37℃in a humidified atmosphere of 5% CO 2 in air. After 24h, daidzein was added at a varied concentration and detected the growth of LoVo cells by MTT method at 2,3,4 and 6 days.RESULTS A dose dependent inhibition of growth of LoVo cells was observed between 5μmol/L to 100 μmol/L, whereas at the concentration below 5 μmol/L, the daidzein could stimulate the growth of LoVo cells.CONCLUSION Daidzein have a biphasic effect on the growth of LoVo cells.

关 键 词：染料木黄酮 大豆苷原 LOVO细胞 结肠癌 癌细胞生长 

分 类 号：R285[医药卫生—中药学] R917[医药卫生—中医学]